DYCP-40C semi-dry blotting system inoshandiswa pamwe chete neelectrophoresis magetsi ekufambisa mapuroteni ari mupolyacrylamide gels pane membrane senitrocellulose membrane, naironi membrane uye PVDF membrane.Semi-dry blotting inoitwa ne graphite plate electrodes mu yakachinjika configuration, sandwiching gel uye membrane pakati pemashizha e-buffer-yakanyoroveswa sefa mapepa anoshanda seion reservoir.Munguva yekuchinja kwe electrophoretic, mamorekuru ane mhosva anokanganisa anotama kunze kwegel uye anoenda kune yakanaka electrode, kwaanoiswa pa membrane.Maelectrodhi eplate, anoparadzaniswa chete nejeli uye sefa mapepa akaturikidzana, anopa simba remunda wepamusoro (V/cm) mhiri kwejeri, richiita kufambisa kwakanyanya, nekukurumidza.Iyo yekutamisa pamusoro peiyo diki DYCP - 40C electrophoresis sero ndeye 150 × 150 (mm), yakakodzera kutamisa akajairwa gels, kusanganisira ayo anobva kuDYCZ-24DN uye DYCZ-24EN electrophoresis cell.
Ngatidzidzei zvimwe nezve mashandiro eiyi semi-dry trans blot apparatus.
Zvishandiso, zviridzwa zvekushandisa DYCP-40C
Electrophoresis power supply DYY-6C, semi-dry trans blot apparatus DYCP-40C, buffer solution, uye midziyo yebuffer solution.etc.
Operation Matanho
1. Isai gel nemagirazi egirazi mumhinduro yekutamisa buffer
2. Edza ukuru hwegel
3.Gadzirira zvidimbu zvitatu zvepepa resefa maererano nehukuru hwejeri, uye saizi yepepa resefa inofanira kunge yakakura zvishoma pane saizi yegel;Pano tinoshandisa Whatman filter paper;
4.Isa zvidimbu zvitatu zvebepa resefa mubuffer solution zvishoma nezvishoma, uye rega bepa resefa rinyudzwe mubuffer zvachose, uye dzivirira kuva nemafuru emhepo;
5.Gadzirira uye cheka nitrocellulose membrane zvichienderana nehukuru hwegel uye sefa bepa;saizi ye nitrocellulose membrane inofanira kunge yakakura kupfuura saizi yegel uye sefa bepa;
6.Isa iyo nitrocellulose membrane mumushonga webuffer;
7.Bvisa zvidimbu zvitatu sefa bepa uye udonhedze imwe buffer solution kusvika pasisina buffer solution inodonha kubva mu membrane;wobva waisa pepa resefa pazasi peDYCP-40C;
8.Tora gel kubva pamahwendefa egirazi, zvinyoro kuchenesa gel stacking, uye uise gel mumushonga webuffer;
9.Isa gel papepa resefa, tanga kubva kune rumwe rutivi rwejeri kuti udzivise mhepo inoputika;
10.Shandisa chishandiso chakakodzera kubvisa mabhubhu emhepo pakati pejeri uye sefa bepa.
11.Ivhara iyo nitrocellulose membrane pane gel, iyo yakaoma side yakananga kujeri.Uye wobva washandisa chishandiso chakakodzera kubvisa mabhuru emhepo pakati pe membrane uye gel.Isa zvidimbu zvitatu zvesefa bepa pane membrane.Zvichiri kudikanwa kushandisa chishandiso chakakodzera kubvisa mabhuru emhepo pakati pebepa resefa uye membrane.
12.Ivhara chivharo, uye isa electrophoresis inomhanya parameters, iyo inogara iripo 80mA;
13.Iyo electrophoresis yakaitwa.Isu tinowana mhedzisiro sekutevera;
Beijing Liuyi Biotechnology Co., Ltd yave nyanzvi mukugadzira zvigadzirwa zve electrophoresis kweanopfuura makore makumi mashanu.Isu tiri ISO9001 & ISO13485 kambani yakasimbiswa uye nyanzvi mukugadzira electrophoresis matangi, magetsi, UV transilluminator, uye gel zvinyorwa & yekuongorora system, zvakadaro, isu tinopa OEM sevhisi kune vatengi vedu, pamwe neODM sevhisi.
Isu tave kutsvaga vatinoshanda navo, vese OEM uye vanogovera vanogamuchirwa.
Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu.Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.
Nguva yekutumira: Kubvumbi-04-2023