Takagovana zvakati wandei svondo rapfuura pakushandisa cellulose acetate membrane electrophoresis, uye isu tichapedzisa iyi nyaya pano nhasi kune yako referensi.
Sarudzo ye Buffer Concentration
Iyo buffer concentration inoshandiswa mu cellulose acetate membrane electrophoresis kazhinji yakaderera pane iyo inoshandiswa mubepa electrophoresis.Iyo inowanzoshandiswa pH 8.6Barbital buffer inowanzosarudzwa mukati mechikamu che 0.05 mol/L kusvika 0.09 mol/L.Pakusarudza kusungirirwa, kutsunga kwekutanga kunoitwa.Semuenzaniso, kana kureba kwe membrane strip pakati pe electrode mu electrophoresis chamber iri 8-10cm, voltage ye 25V inodiwa pa centimita yehurefu hwe membrane, uye ikozvino kusimba kunofanira kuva 0.4-0.5 mA pa centimita yehupamhi hwe membrane.Kana izvi zvakakosha zvisina kuwanikwa kana kudarika panguva ye electrophoresis, iyo buffer concentration inofanira kuwedzerwa kana kuderedzwa.
Iyo yakanyanya kuderera buffer concentration inozokonzera kukurumidza kufamba kwemabhandi uye kuwedzera kwebhendi upamhi.Nekune rimwe divi, yakanyanya kukwirisa buffer concentration inononoka kutama kwebhendi, zvichiita kuti zviome kusiyanisa mamwe mabhendi ekuparadzanisa.
Izvo zvinofanirwa kucherechedzwa kuti mu cellulose acetate membrane electrophoresis, chikamu chakakosha chezvino chinoitwa kuburikidza nemuenzaniso, izvo zvinogadzira huwandu hwakawanda hwekupisa.Dzimwe nguva, iyo yakasarudzwa buffer concentration inogona kunzi yakakodzera.Nekudaro, pasi pemamiriro ekuwedzera tembiricha yezvakatipoteredza kana kana uchishandisa yakakwira voltage, kubuda kwemvura nekuda kwekupisa kunogona kuwedzera, zvichikonzera kuwandisa kwakanyanya kwebuffer uye kutokonzera kuti membrane iome.
Muenzaniso Vhoriyamu
Mu cellulose acetate membrane electrophoresis, huwandu hwesample vhoriyamu hunotarwa nezvinhu zvakasiyana, zvinosanganisira electrophoresis mamiriro, zvimiro zvesample pachayo, nzira dzekusvibisa, uye maitiro ekuona.Seyakajairwa musimboti, iyo inonyanya kutarisisa nzira yekuona, iyo idiki vhoriyamu yemuenzaniso inogona kuva, iyo inobatsira pakuparadzana.Kana vhoriyamu yemuenzaniso yakawandisa, maitiro ekuparadzanisa e electrophoretic anogona kunge asina kujeka, uye kusvibisa kunogona zvakare kutora nguva.Nekudaro, kana uchiongorora huwandu hwakapatsanurwa mabhendi uchishandisa elution colorimetric yekuona nzira, iyo sampuli vhoriyamu haifanirwe kuve diki zvakanyanya, sezvo inogona kuguma yakaderera yekunyudza kukosha kune zvimwe zvikamu, zvichitungamira kune zvikanganiso zvakanyanya mukuverenga zvirimo.Mumamiriro ezvinhu akadaro, vhoriyamu yemuenzaniso inofanira kuwedzerwa zvakakodzera.
Kazhinji, vhoriyamu yemuenzaniso yakawedzerwa pane imwe neimwe centimita yemuenzaniso wekushandisa mutsara unotangira kubva ku0.1 kusvika ku5 μL, yakaenzana nechiyero chemuenzaniso che5 kusvika ku1000 μg.Semuenzaniso, mune yenguva dzose serum protein electrophoresis kuongororwa, vhoriyamu yemuenzaniso yakawedzerwa pane imwe neimwe centimita yemutsetse wekushandisa kazhinji haipfuure 1 μL, yakaenzana ne60 kusvika 80 μg yemapuroteni.Nekudaro, kana uchiongorora lipoproteins kana glycoproteins uchishandisa imwecheteyo electrophoresis nzira, iyo sampuli vhoriyamu inoda kuwedzera zvinoenderana.
Mukupedzisa, iyo yakanyanya kufanirwa sampuli vhoriyamu inofanirwa kusarudzwa zvichienderana nemamiriro chaiwo kuburikidza neakatevedzana ekutanga kuyedza.
Kusarudzwa kweStaining Solution
Iwo mabhendi akapatsanurwa mu cellulose acetate membrane electrophoresis anowanzo kusvibiswa asati aonekwa.Zvikamu zvakasiyana zvemuenzaniso zvinoda nzira dzakasiyana dzekusvibisa, uye nzira dzekusvibisa dzakakodzera cellulose acetate membrane electrophoresis dzinogona kusashanda zvachose kusefa bepa.
Pane misimboti mitatu mikuru yekusarudza staining solution yecellulose acetate membrane.Chekutanga,Madhayi anonyungudika nemvura anofanirwa kusarudzwa pane dhayi-anonyungudika nedoro kudzivirira membrane shrinkage uye deformation inokonzerwa neiyo yekupedzisira inosvibisa mhinduro.Mushure mekusvibisa, zvakakosha kushambidza membrane nemvura uye kuderedza nguva yekusvibisa.Zvikasadaro, membrane inogona kupetwa kana kutetepa, izvo zvingakanganisa kucherechedzwa kunotevera.
Chechipiri, zviri nani kusarudza madhayi ane yakasimba staining affinity yemuenzaniso.Mu cellulose acetate membrane electrophoresis yemaprotein e serum, amino dema 10B inowanzoshandiswa nekuda kwesimba rayo rekusvibisa hukama hweakasiyana serum protein components uye kugadzikana kwayo.
Chechitatu, dhayi dzemhando dzakavimbika dzinofanirwa kusarudzwa.Mamwe madhayi, kunyangwe aine zita rimwe chete, anogona kunge aine tsvina inokonzeresa kune yakasviba kumashure mushure mekusvibisa.Izvi zvinogona kunyange kudzima mabhendi akapatsanurwa pakutanga, zvichiita kuti zviome kusiyanisa.
Chekupedzisira, kusarudzwa kwekusvibisa mhinduro yekufungisisa kwakakosha.Nemasikirwo, zvingaite sekuti yakakwira staining solution yekumisikidza yaizotungamira kune yakawedzera kusvibiswa kwezvikamu zvemuenzaniso uye zvirinani mhedzisiro.Zvisinei, izvi hazvisizvo.Iyo inosunga hukama pakati pezvikamu zvemuenzaniso uye dhayi ine imwe muganhu, iyo isingawedzere nekuwedzera kwekusvibisa kugadzirisa kugadzirisa.Pane zvinopesana, yakanyanyisa yakakwirira staining solution concentration haingotambisi dhayi chete asiwo inoita kuti zviome kuwana mamiriro akajeka.Uyezve, kana kusimba kweruvara kwasvika pahumwe hukuru hunokosha, kunyura kwedhayi hakuteveri hukama hwemutsara, kunyanya mumiyero yehuwandu.
Ruzivo rwekuziva nezveBeijing Liuyi Biotechnology's cellulose acetate membraneelectrophoresis tank uye yayo electrophoresis application, ndapota shanya pano:
lKuedza kupatsanura serum protein neCellulose Acetate Membrane
lCellulose Acetate Membrane Electrophoresis
Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu.Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.
ReferenceElectrophoresis(Chinyorwa chechipiri) naVaLi
Nguva yekutumira: Jun-06-2023