DNA gel electrophoresis inzira yakajairika yemamorekuru biology inoshandiswa kupatsanura uye kuongorora zvidimbu zveDNA zvichienderana nehukuru hwazvo.Kuita uku kunosanganisira kurodha zvimedu zveDNA zvakakura zvakasiyana-siyana pajeri rakagadzirwa neagarose, kabhohaidhiretsi inowanikwa mualgae dzvuku.
Kugadzirira uye kukanda gel agarose
- Dissolve agarose muhuwandu hwakakodzera hwe electrophoresis buffer.Iko kusanganiswa kwejeri kunotarirwa nehuwandu-kusvika-vhoriyamu reshiyo, senge 1g yeagarose mu100.ml ye buffer ye1% gel.
- Pisa musanganiswa mu microwave, uchitenderedza mudziyo kuti uone kupera kwakakwana kweagarose.
- Wedzera ethidium bromide kune gel mhinduro kune yekupedzisira kusanganiswa kwe 0.5mg/ ml.Ethidium bromide inopindirana pakati pepedyo DNA base pairs uye inoburitsa orenji fluorescence pasi peUV mwenje.Ziva kuti ethidium bromide icarcinogen, saka kuibata kunoda matanho ekuchengetedza akakodzera sekupfeka magirovhosi.
- Kutonhodza mushonga wegel mumvura yekugezesa kuti udzivise tirayi yegel kubva pakurwisana nekuda kwekupisa kwakanyanya.
- Isa muzinga mumushonga wejeri kuti ugadzire matsime ekuenzanisa.Sarudza mizinga inokodzera huwandu hwesample yeDNA yauchange uchirodha.Dururirai agarose gel solution mukatigel trayuye bvumira kuti iome pakupisa kwekamuri.
- Kana gel raoma, bvisa muzinga.Kana usiri kuzoshandisa gel nekukasika, peta mupurasitiki yekuputira woichengeta pa4 ℃ kusvika pazvinenge zvichidiwa.
Kugadzirira uye kushandisa gel
Usati watanga electrophoresis, sanganisa sampuli yeDNA nekurodha buffer.Iyo buffer yekurodha kazhinji katanhatu yakawedzera kudzika uye inobatsira sampuli kunyura pasi pematsime uye kuronda mafambiro panguva ye electrophoresis.
Isa simba rekupa kune yakataurwa voltage.
Wedzera yakakwana electrophoresis buffer kune gel tank kuti ivhare nzvimbo yegel.Iva nechokwadiiyo chaiyo electrode kubatana.
Isa sampuli yeDNA uye mamorekuru ekuyera mamaki mumatsime ejeri.
Batidza magetsi kuti utange electrophoresis.
Kucherechedza zvidimbu zveDNA zvakaparadzana
Mushure me electrophoresis, bvisa magetsi uye bvisa gel.
Shandisa UV chiedza sosi kuvhenekera gel;Zvimedu zveDNA zvichaonekwa semaranjisi efluorescent mabhendi.
Nyora mufananidzo wegel kuti uongorore zvidimbu zveDNA zvakaparadzaniswa.
Mushure mekuedza, ita shuwa kuraswa kwakaringana kwegel uye electrophoresis buffer, uchitevera gwara rerabhoritari.Gara wakapfeka magirovhosi paunenge uchibata gels uye mabhafa ane ethidium bromide kudzivirira kubva pakusangana nechinhu chine njodzi ichi.
DNA gel electrophoresis inoshandiswa zvakanyanya mumolecular biology uye genetics tsvakiridzo yekufungidzira DNA mamorekuru saizi, kupatsanura zvidimbu zveDNA, kuona shanduko dzemajini, uye DNA kudhindisa zvigunwe, pakati pezvimwe zvinoshandiswa.Iri nyore uye rinoshanda nzira yekuyedza iyo inobatsira mukunzwisisa kuumbwa uye hunhu hweDNA samples.
Beijing Liuyi Biotechnology Co.Ltd, inyanzvi inogadzira inotarisa pane zvigadzirwa zve electrophoresis makore anopfuura makumi mashanu, inopa huwandu hwematangi e electrophoresis (makamuri / masero) egel electrophoresis, senge horizontal electrophoresis matangi (makamuri / masero)DNAgel electrophoresis, uye vertical electrophoresis matangi (makamuri/masero) eprotein gel electrophoresis.Zvichakadaro, inopawo akasiyana marudzi e electrophoresis magetsi emagetsi, kabati rima bhokisi analyzer uye UV transilluminator.Unogona kusarudza Beijing Liuyi's electrophoresis matangi (makamuri/masero) kuyera huremu hweRNA uye kupatsanura RNA yemasikisi akasiyana erabhoritari yako.
Pano tichadarokurudziraimwe mhando ye horizontal electrophoresis tank (chamber/cell) modhiDYCP-31DN kugadzira uye kumhanya gel.
Mushure mekumhanyisa gel, unogona kushandisa yedu gel mufananidzo & yekuongorora system modhiWD-9413Bkutarisa, kuongorora uye kutora mapikicha ejeri.Liuyi Biotech inopawo UV transilluminator (UV Analyzer) kuona iyo gel.Tine dema-bhokisi mhando UV transilluminator (UV Analyzer) modhiWD-9403A,9403C,WD-9403F, inotakurika UV transilluminator (UV Analyzer) modhiWD-9403Buye kubata UV transilluminator (UV Analyzer)WD-9403Enokuti unosarudza.
Iyo Beijing Liuyi mhando ine anopfuura makore makumi mashanu nhoroondo muChina uye kambani inogona kupa yakagadzikana uye yemhando yepamusoro zvigadzirwa pasi rese.Kupfuura makore ebudiriro, yakakodzera sarudzo yako!
Isu tave kutsvaga vanobatika, vese OEM electrophoresis tank uye vanogovera vanogamuchirwa.
Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu.Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.
Nguva yekutumira: Aug-08-2023