Gel electrophoresis ndiyo imwe yenzira huru dzinoshandiswa mumolecular biology pakuongorora DNA.Iyi nzira inosanganisira kufamba kwezvidimbu zveDNA kuburikidza nejeri, uko zvinoparadzaniswa zvichienderana nekukura kana chimiro.Zvisinei, wakambosangana nezvikanganiso panguva yekuedza kwako kwe electrophoresis, zvakadai semabhandi akaiswa pane agarose gel, kana kuti pasina mabhandi pane gel?Chii chingava chikonzero chezvikanganiso izvi?
Vanamazvikokota vedu vakapfupikisa vakaroorana vekugadzirisa dambudziko pano kuti utarise iwe.
1. Mabhandi akaiswa paagarose gel
●DNA yakaderedzwa.Dzivisa kusvibiswa kwenyuclease.
● The electrophoresis buffer haisi itsva.Mushure mekudzokorora kushandiswa kwe electrophoresis buffer, simba reionic rinoderera, uye pH yaro inowedzera, saka buffer simba rinopera, izvo zvinokanganisa electrophoresis effect.Inokurudzirwa kutsiva electrophoresis buffer kakawanda.
● Mamiriro asina kunaka e electrophoresis akashandiswa.Usabvumira magetsi kudarika 20 V / cm, uye chengetedza tembiricha <30 ° C panguva ye electrophoresis.Kune hofori yeDNA strand electrophoresis, tembiricha inofanira kuva <15° C. Tarisa kuti electrophoresis buffer ine buffer capacity yakakwana.
● DNA yakawandisa yakaiswa pajeri.Deredza huwandu hweDNA.
● Munyu wakawandisa muDNA.Shandisa ethanol precipitation kuti ubvise munyu wakawandisa munguva yepamusoro.
● DNA yakanga ine mapuroteni.Shandisa phenol extractions kubvisa mapuroteni ari mberi.
● DNA yakachinjwa.Usapisa pamberi pe electrophoresis.Dilute DNA mubuffer ine 20 mM NaCl.
2. Anomalies DNA band migration
● Kudzorerwazve kweCOS saiti ye λHind III chidimbu.Pisa DNA kwemaminitsi mashanu pasi pe 65° C pamberi pe electrophoresis, wozoitonhodza paaizi unit kwemaminitsi mashanu.
● Mamiriro asina kunaka e electrophoresis akashandiswa.Usabvumira magetsi kudarika 20 V / cm, uye chengetedza tembiricha <30 ° C panguva ye electrophoresis.Tarisa iyo electrophoresis buffer ine yakakwana buffer kugona.
● DNA yakachinjwa.Usapisa pamberi pe electrophoresis.Dilute DNA mubuffer ine 20 mM NaCl.
3. Kufenda kana kusakwana mabhandi eDNA pane agarose gel
● Pakanga pasina huwandu hwakakwana kana huwandu hweDNA yakaiswa pagel.Wedzera huwandu hweDNA.Polyacrylamide gel electrophoresis inonzwa zvishoma pane agarose electrophoresis, uye sampuli kurodha inogona kudzikiswa zvakakodzera.
● DNA yakaderedzwa.Dzivisa kusvibiswa kwenyuclease.
● DNA yakaitwa nemagetsi kubva pagel.Electrophorese gel kwenguva shoma, shandisa yakaderera voltage, kana kushandisa yakakwira muzana gel.
● Wlight source isina kunaka yakashandiswa pakuona kweethidium bromide-stained DNA.Shandisa shortwavelength (254 nm) W mwenje kuti unzwe zvakanyanya.
4. Mabhandi eDNA asipo
●Iyo diki saizi DNA yaive electrophoresed kubva gel.Electrophorese gel kwenguva shoma, shandisa yakaderera voltage, kana kushandisa yakakwira muzana gel.
● Zvakaoma kusiyanisa mabhendi eDNA emamorekuru akafanana.Wedzera nguva ye electrophoresis, uye tarisa kusungirirwayegel kuti ive nechokwadi chechokwadi muzana gel rinoshandiswa.
● DNA yakachinjwa.Usapisa pamberi pe electrophoresis.Dilute DNA mubuffer ine 20 mM NaCl.
● Tambo dzeDNA dzakakura, uye gel electrophoresis yakajairika haina kukodzera.Ongorora pane pulse gel electrophoresis.Ndedzipi dzimwe nyaya dzawakambove nadzo neagarose gel electrophoresis?Tichatsvagisa zvimwe zvemadhiraivha mune ramangwana.
Beijing Liuyi biotechnology Co., Ltd (Liuyi Biotech) ikambani yakasarudzika inotarisisa zvigadzirwa zvine chekuita ne electrophoresis muChina.Nyaya yaro inotanga muna 1970 apo China yakanga isati yapinda mukugadzirisa uye kuvhura nguva.Kuburikidza nebudiriro yemakore, Liuyi Bitotech ine mhando yayo, inozivikanwa seLiuyi Brand mumusika wepamba wezvigadzirwa zve electrophoresis.
Iyo Liuyi mhando ine inopfuura makore makumi mashanu nhoroondo muChina uye kambani inogona kupa yakagadzikana uye yemhando yepamusoro zvigadzirwa pasi rese.Kuburikidza nekukura kwemakore, yakakodzera sarudzo yako!
Iwo horizontal electrophoresis masero (matangi/makamuri) eLiuyi Biotech ane mhando yepamusoro ane chitarisiko chakanaka.Nemasaizi akasiyana ematireyi egel, anogona kusangana neako akasiyana ekuedza zvinodiwa.Tine yedu tekinoroji timu uye fekitori.Kubva pakugadzira kusvika pakugadzira, zvigadzirwa kusvika kuzvikamu zvakakosha, tinogona kudzora maitiro ese.Iyo DYCP 31 yakatevedzana ndeye DNA electrophoresis, ari modhiDYCP-31BN, DYCP-31CN,DYCP-31DN,uyeDYCP-31E.Misiyano pakati pavo ndeye gel saizi uye mutengo.Isu tinopa hukuru hwakazara hwezvigadzirwa kune vatengi vedu.MuenzanisoDYCP-32Cinogona kuita yakakura gel 250mm * 250mm.
Zvichakadaro, isu tinokurudzira yedu electrophoresis magetsiDYY-6C,DYY-6DuyeDYY-10Cnokuda kwemasero edu e electrophoresis (matangi / makamuri) DYCP-31 uye 32 series.
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Nguva yekutumira: Chivabvu-09-2022