Pulsed Field Gel Electrophoresis CHEF Mapper A6
Zvinotsanangurwa
| Model | CHEF Mapper A6 |
| Voltage Gradient | 0.5V/cm kusvika 9.6V/cm, yakawedzerwa ne0.1V/cm |
| Maximum Current | 0.5A |
| Maximum Voltage | 350V |
| Pulse Angle | 0-360° |
| Nguva Gradient | Linear |
| Kuchinja Nguva | 50ms kusvika 18h |
| Maximum Kumhanya Nguva | 999h |
| Nhamba yeElectrodes | 24, inodzorwa yakazvimirira |
| Multi-State Vector Shanduko | Inotsigira anosvika gumi vheji pa pulse cycle |
| Temperature Range | 0 ℃ kusvika 50 ℃, yekuona kukanganisa <± 0.5 ℃ |
Tsanangudzo
Pulsed field gel electrophoresis (PFGE) inopatsanura mamorekuru eDNA nekuchinjanisa munda wemagetsi pakati pema electrode akasiyana epamhepo, zvichikonzera mamorekuru eDNA, ayo anogona kunge ari mamirioni emapazi maviri akareba kuti adzoke uye afambe kuburikidza neagarose gel pores nekumhanya kwakasiyana. Inowana kugadziriswa kwepamusoro mukati mehuwandu uhwu uye inonyanya kushandiswa mukugadzira biology; kuzivikanwa kweiyo biological uye microbial lineages; tsvakurudzo mune molecular epidemiology; zvidzidzo zvezvikamu zvakakura zveplasmid; kugarisana kwemajini echirwere; mepu yemuviri yemajini, kuongororwa kweRFLP, uye DNA kudhindisa zvigunwe; tsvakurudzo yekufa kwesero yakarongwa; zvidzidzo pamusoro pekukuvara kweDNA uye kugadzirisa; kuparadzaniswa uye kuongorora genomic DNA; kupatsanurwa kwechromosomal DNA; kuvaka, kuzivikanwa, uye kuongororwa kwemahombe-fragment genomic library; uye transgenic research.t concentrations yakaderera se0.5 ng/µL (dsDNA).
Application
Inokodzera kuona uye kupatsanura mamorekuru eDNA kubva pa100bp kusvika 10Mb muhukuru, inowana kugadziriswa kwepamusoro mukati merudzi urwu.
Feature
• Advanced Technology: Inosanganisa CHEF nePACE pulsed-field technologies kuti iwane mibairo yakakwana nemigwagwa yakatwasuka, isina kubhenda.
• Kuzvimiririra Kudzora: Zvimiro 24 zvakazvimiririra zvinodzorwa platinum electrodes (0.5mm dhayamita), ine electrode imwe neimwe inotsiviwa yega.
• Automatic Calculation Function: Inobatanidza zvakasiyana-siyana zvakakosha zvakadai sevoltage gradient, tembiricha, kona yekuchinja, nguva yekutanga, nguva yekupedzisira, nguva yekuchinja ikozvino, nguva yekumhanya, voltage, uye ikozvino yekuverenga otomatiki, kubatsira vashandisi kuwana mamiriro ekuedza akakwana.
• Unique Algorithm: Inoshandisa yakasarudzika pulse control algorithm kune zviri nani kupatsanurwa mhedzisiro, nyore kusiyanisa pakati pemutsara uye denderedzwa DNA, nekuwedzerwa kuparadzaniswa kwehukuru denderedzwa DNA.
• Chirongwa Chekuchengetedza: Inochengetedza kusvika ku15 yakaoma yekuedza mapurogiramu, imwe neimwe inosanganisira isingasviki 8 purogiramu modules.
• Multi-State Vector Change: Inotsigira kusvika ku10 vectors per pulse cycle, zvichibvumira tsanangudzo yekona imwe neimwe, voltage, uye nguva.
• Transition Slope: Linear, concave, kana convex uchishandisa hyperbolic mabasa.
• Automation: Inorekodha uye inotangazve electrophoresis kana chirongwa chacho chikavhiringidzwa nekuda kwekushaya simba.
• Mushandisi-Anogadzirika: Inobvumira vashandisi kumisa yavo mamiriro.
• Flexibility: Iyo system inogona kusarudza chaiyo voltage gradients uye nguva dzekuchinja kune mamwe masaizi eDNA.
• Sikirini Yakakura: Yakashongedzerwa ne7-inch LCD skrini yekushanda nyore, inoratidzira yakasarudzika software yekutonga kwekushandisa nyore uye nyore.
• Kuona Tembiricha: Maviri etembiricha anoongorora tembiricha anoona zvakananga tembiricha yebhafa ine kukanganisa kuri pasi pe ± 0.5 ℃.
• Circulation System: Inouya ne-buffer circulation system inonyatso kudzora uye inotarisa tembiricha yebuffer solution, kuve nechokwadi chekushisa nguva dzose uye ionic balance panguva ye electrophoresis.
• Kuchengetedzwa Kwepamusoro: Kunosanganisira spaparent acrylic safety cover iyo inodimbura otomatiki magetsi kana yasimudzwa, pamwe chete nezvakawandisa uye zvisina-mutoro kudzivirira mabasa.
• Adjustable Leveling: Tangi ye electrophoresis uye gel caster inoratidzira tsoka dzinogadziriswa kuti dzienzane.
• Mold Design: Tangi ye electrophoresis inogadzirwa ne-integrated mold structure pasina kusungirirwa; iyo electrode rack yakashongedzerwa ne 0.5mm platinamu electrodes, kuve nechokwadi kusimba uye yakagadzikana mhedzisiro yekuedza.
• Pulse angle: The pulse angle inogona kusarudzwa zvakasununguka pakati pe 0-360 °, zvichiita kuti vashandisi vawane kupatsanurwa kunobudirira kubva kuchromosomal hombe kusvika kudiki plasmid DNA mukati megadziriro imwechete.
• Pulse Time Gradient: Inosanganisira mutsara uye isina-mutsara (convex uye concave) pulse time gradients. Non-linear gradients inopa kupatsanurwa kwakakura kwesimba renji, zvichibvumira vashandisi kunyatsoona zvidimbu zvezvidimbu.
• Real-time Monitoring: Panguva imwe chete inoratidza zvigadziro zvekugadzirisa uye mamiriro ekushanda, anowirirana ne-real-time monitoring software.
• Sekondari Pulses: Iyo yechipiri pulse tekinoroji inogona kukurumidza kuburitswa kweDNA kubva kune agarose gel, kuita kuti kuparadzaniswa kwezvimedu zvakakura kwazvo zveDNA uye kugadzirisa kugadzirisa.
• Inoenderana nePulseNet China: Iyo sisitimu inogona kuwirirana neyenyika pathogen monitoring network uye PulseNet China yekutarisisa network, ichibvumira kusiyanisa zvidimbu zvine huremu hwemamorekuru hwakafanana.
FAQ
Mubvunzo: Chii chinonzi Pulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis inzira inoshandiswa kupatsanura mamorekuru eDNA anoenderana nekukura kwawo. Inosanganisira kushandura nzira yemunda wemagetsi mune gel matrix kuti igone kupatsanurwa kwezvidimbu zveDNA zvakakurisa kuti zvigadziriswe neagarose gel electrophoresis.
Q: Ndeapi mashandisirwo ePulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis inoshandiswa zvakanyanya mumolecular biology uye genetics ye:
Mepu yemamorekuru makuru eDNA, akadai semakromosome nemaplasmids.
• Kusarudza hukuru hwejenome.
• Kudzidza kusiyana kwemajini uye hukama hwekushanduka-shanduka.
• Molecular epidemiology, kunyanya yekutevera kuputika kwechirwere chinotapukira.
• Ongororo yekukuvara kweDNA uye kugadzirisa.
• Kuona kuvepo kwemajini chaiwo kana kutevedzana kweDNA.
Mubvunzo: Pulsed Field Gel Electrophoresis inoshanda sei?
A: Pulsed Field Gel Electrophoresis inoshanda nekuisa DNA mamorekuru kune pulsed electric field inochinjana munzira. Izvi zvinobvumira mamorekuru eDNA kuti azvigadzirise pakati pemapulses, achigonesa kufamba kwavo kuburikidza negel matrix. Madiki mamorekuru eDNA anofamba nekukurumidza kuburikidza nejeri, nepo makuru anofamba zvishoma nezvishoma, achibvumira kuparadzaniswa kwavo zvichienderana nehukuru.
Q: Ndeipi musimboti uri shure kwePulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis inoparadzanisa mamorekuru eDNA zvichienderana nekukura kwawo nekudzora nguva uye kutungamirirwa kwemagetsi emunda wemagetsi. Iyo inochinjika munda inokonzeresa mamorekuru eDNA kuti arambe achizvirongedza, zvichitungamira kutama kwavo kuburikidza nejel matrix uye kupatsanurwa zvichienderana nehukuru.
Mubvunzo: Ndezvipi zvakanakira Pulsed Field Gel Electrophoresis?
A: High resolution yekuparadzanisa mamorekuru makuru eDNA anosvika miriyoni imwe base pairs.Kugona kugadzirisa uye kusiyanisa zvidimbu zveDNA zvehukuru hwakafanana.Kusiyana-siyana mukushandiswa, kubva ku microbial typing kusvika ku molecular genetics uye genomics.Nzira yakasimbiswa yezvidzidzo zve epidemiological uye genetic mepping.
Q: Ndeipi midziyo inodiwa kuPulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis inowanzoda electrophoresis apparatus ine maelectrodes akasarudzika ekugadzira minda inopuruzira. Agarose gel matrix ine kutarisisa kwakakodzera uye buffer. Simba rekushandisa rinokwanisa kugadzira high-voltage pulses.Cooling system kuti iparadze kupisa kunogadzirwa panguva ye electrophoresis, uye pombi inotenderera.
