PCR Thermal Cycler WD-9402M
Zvinotsanangurwa
| Model | WD-9402M |
| Capacity | 96×0.2ml |
| Tube | 96x0.2ml (PCR ndiro isina/semi siketi), 12x8x0.2ml mitsetse, 8x12x0.2ml mitsetse, 0.2ml machubhu (kureba 20 ~ 23mm) |
| Block Temperature Range | 0-105 ℃ |
| Block Temperature Accuracy | ±0.2℃ |
| Block Temperature Uniformity | ±0.5℃ |
| Heating Up Rate (avhareji) | 4℃ |
| Kutonhodza pasi Mwero (avhareji) | 3℃ |
| Temperature Control | Block/Tube |
| Gradient Temp. Range | 30-105 ℃ |
| Max.Heating Rate | 5℃/s |
| Max.Kutonhodza Mwero 4.5℃ /S | 4.5℃/s |
| Gradient Set Span | Max. 42℃ |
| Gradient Temperature Accuracy | ±0.3℃ |
| Tembiricha inoratidza huchokwadi | 0.1℃ |
| Heating Lid Temperature range | 30 ℃ ~ 110 ℃ |
| Automatically Heating Lid | Dzima otomatiki kana sampuro yakaderera pane 30 ℃ kana chirongwa chapfuura |
| Timer Kuwedzera / Kuderera | -599~599 S yeYakareba PCR |
| Tembiricha Kuwedzera / Kuderera | -9.9~9.9℃ yeTouchdown PCR |
| Timer | 1s~59min59sec/ Zvisingaperi |
| Zvirongwa zvakachengetwa | 10000+ |
| Max.Cycles | 99 |
| Max.Matanho | 30 |
| Pause Function | Ehe |
| Touchdown Basa | Ehe |
| Yakareba PCR Basa | Ehe |
| Mutauro | Chirungu |
| Chirongwa Kumbomira Basa | Ehe |
| 16 ℃ Tembiricha Kubata Basa | Zvisingaperi |
| Chaiyo-nguva yekushanda mamiriro | Mufananidzo-mavara aratidzwa |
| Kukurukurirana | USB 2.0 |
| Dimensions | 200mm×300mm×170mm (W×D×H) |
| Kurema | 4.5kg |
| Magetsi | 100-240VAC , 50/60Hz, 600W |
Tsanangudzo
Thermal cycler inoshanda nekudzokorodza kupisa nekutonhodza reaction musanganiswa ine DNA kana RNA template, primers, uye nucleotides. Iyo tembiricha yebhasikoro inodzorwa chaizvo kuti iwane inodiwa denaturation, annealing, uye ekuwedzera matanho ePCR maitiro.
Kazhinji, inopisa inotenderera ine chivharo chine matsime akawanda kana machubhu panoiswa reaction musanganiswa, uye tembiricha mutsime rega rega inodzorwa yakazvimirira. Iyo block inodziiswa uye inotonhodzwa uchishandisa Peltier element kana imwe yekudziya uye kutonhora system.
Mazhinji anopisa mabhasikoro ane mushandisi-ane hushamwari interface inobvumira mushandisi kuronga uye kugadzirisa ma paramita eku bhasikoro, senge tembiricha ye annealing, nguva yekuwedzera, uye nhamba yematenderedzwa. Vanogona zvakare kuve nechiratidziro chekutarisa mafambiro ekuita, uye mamwe mamodheru anogona kupa maficha epamberi senge gradient tembiricha yekudzora, akawanda block masisitimu, uye kure kutarisa uye kutonga.
Application
Polymerase Chain Reaction (PCR) inzira yemamorekuru biology inoshandiswa zvakanyanya kune akasiyana maapplication. Mamwe maapplication akajairika ePCR anosanganisira:
DNA Amplification: Chinangwa chikuru chePCR ndechekukudza kutevedzana kweDNA. Izvi zvakakosha pakuwana huwandu hwakakwana hweDNA kuti uwedzere ongororo kana zviedzo.
Genetic Testing: PCR inoshandiswa zvakanyanya mukuongororwa kwemajini kuti ione genetic markers kana shanduko dzine chekuita nezvirwere. Izvo zvakakosha pazvinangwa zvekuongorora uye kudzidza genetic predispositions.
DNA Cloning: PCR inoshandiswa kugadzira huwandu hukuru hwechimedu cheDNA, icho chinogona kugadzirwa muvekita kuti iwedzere kunyengedza kana kuongororwa.
Forensic DNA Ongororo: PCR yakakosha musainzi yeforensic yekusimudzira maminetsi eDNA samples anowanikwa kubva munzvimbo dzematsotsi. Inobatsira mukuziva vanhu uye nekumisikidza hukama hwemajini.
Microbial Detection: PCR inoshandiswa kuona hutachiona hwehutachiona mumakiriniki samples kana sampuli dzezvakatipoteredza. Inobvumira kukurumidza kuzivikanwa kwehutachiona hwehutachiona.
Quantitative PCR (qPCR kana Real-Time PCR): qPCR inogonesa kuverengerwa kweDNA panguva yekusimudzira maitiro. Inoshandiswa kuyera mazinga ekutaura kwemajini, kuona huwandu hwehutachiwana, uye kuenzanisa huwandu hweakatevedzana eDNA.
Molecular Evolution Zvidzidzo: PCR inoshandiswa muzvidzidzo kuongorora kusiyana kwemajini mukati mehuwandu, hukama hwekushanduka-shanduka, uye phylogenetic analysis.
Environmental DNA (eDNA) Ongororo: PCR inoshandiswa kuona kuvapo kwezvimwe zvipenyu mumasampuli ezvakatipoteredza, zvichibatsira mukusiyana-siyana kwezvipenyu uye zvidzidzo zvezvakatipoteredza.
Genetic Engineering: PCR chishandiso chakakosha muinjiniya yemajini kuunza chaiyo DNA kutevedzana mune zvipenyu. Inoshandiswa pakusikwa kwezvipenyu zvine genetically modified organisms (GMOs).
Kuteedzera Kugadzirira Raibhurari: PCR inobatanidzwa mukugadzirira kwemaraibhurari eDNA kune inotevera-chizvarwa kutekisheni matekinoroji. Inobatsira kukwidziridza zvidimbu zveDNA zvekudzika kutevedzana kwekushandisa.
Site-Directed Mutagenesis: PCR inoshandiswa kuunza shanduko chaiyo muDNA sequences, zvichibvumira vatsvakurudzi kuti vadzidze migumisiro yeimwe genetic shanduko.
DNA Fingerprinting: PCR inoshandiswa muDNA mafingerprinting matekiniki ekuzivikanwa kwemunhu, kuyedzwa kwababa, uye kutanga hukama hwebhayoloji.
Feature
•Chitarisiko chakanaka, compact size, uye tight structure.
• Yakashongedzerwa nepamusoro-inoshanda, yakanyarara axial-flow fan kuti iite yakanyarara maitiro ekushanda.
•Inoratidza basa rakakura re 30 ℃, richibvumira kukwenenzvera kwemamiriro ekuedzwa kuti asangane nekukasira kwekuyedza zvinodiwa.
•5-inch high-definition color touchscreen for intuitive and easy operation, inogonesa kushanda nesimba kugadzirisa, kuchengetedza, uye kushanda kwezvirongwa.
•Industrial-giredhi inoshanda sisitimu, inofambisa inoenderera uye isina kukanganisa-isina kushanda 7x24.
•Kukurumidza kuendesa data ku USB flash drive kuti ive nyore chirongwa chekuchengetedza, inosimudzira kuchengetedza data.
• Yepamberi semiconductor yekutonhodza tekinoroji uye yakasarudzika PID tembiricha yekudzora tembiricha inosimudzira kushanda kwese kusvika kune hurefu hutsva: yakakwirira tembiricha kurongeka, kukurumidza kupisa uye kutonhora mitengo, uye zvakafanana kugoverwa module tembiricha.
FAQ
Mubvunzo: Chii chinonzi thermal cycler?
A: A thermal cycler mudziyo werabhoritari unoshandiswa kukwidziridza DNA kana RNA sequences kuburikidza ne polymerase chain reaction (PCR). Inoshanda nekuchovha bhasikoro kuburikidza nenhevedzano yekushanduka kwetembiricha, ichibvumira kutevedzana kweDNA kukwidziridzwa.
Mubvunzo:Ndezvipi zvikamu zvikuru zve thermal cycler?
A: Izvo zvikamu zvikuru zve thermal cycler zvinosanganisira chivharo chekudziya, thermoelectric cooler, tembiricha sensors, microprocessor, uye control panel.
Mubvunzo: Inoshanda sei bhasikoro rinopisa?
A: A thermal cycler anoshanda nekudziya nekutonhodza DNA samples munhevedzano yetembiricha kutenderera. Nzira yebhasikoro inosanganisira denaturation, annealing, uye nhanho dzekuwedzera, imwe neimwe ine tembiricha yakatarwa uye nguva. Aya madenderedzwa anobvumira kutevedzana kweDNA kuti kukwidziridzwe kuburikidza ne polymerase chain reaction (PCR).
Mubvunzo: Ndezvipi zvakakosha zvekufunga nezvazvo pakusarudza thermal cycler?
A: Zvimwe zvinhu zvakakosha zvekufunga nezvazvo pakusarudza chitenderedzwa chekupisa zvinosanganisira huwandu hwematsime kana machubhu ekuita, tembiricha yemhando uye kumhanya kwerampu, iko kurongeka uye kufanana kwekudzora tembiricha, uye mushandisi interface uye software kugona.
Mubvunzo: Unochengetedza sei bhasikoro rinopisa?
A: Kuti uchengetedze bhaisikopo rinopisa, zvakakosha kugara uchichenesa chivharo chekudziya uye machubhu ekuita, tarisa kusakara uye kubvaruka pazvikamu, uye kugadzirisa tembiricha sensors kuti ive nechokwadi chekudzora tembiricha. Izvo zvakakoshawo kutevedzera mirairo yemugadziri wekugara uchigadzirisa uye kugadzirisa.
Mubvunzo: Ndeapi mamwe matanho akajairika ekugadzirisa dambudziko kune anopisa cycler?
A: Mamwe matanho akajairika ekugadzirisa dambudziko remudhudhudhu anopisa anosanganisira kutarisa zvinhu zvakasununguka kana zvakakanganisika, kuona tembiricha yakakodzera uye nguva, uye kuyedza machubhu ekuita kana ndiro kuti ane tsvina kana kukuvara. Izvo zvakakoshawo kutarisa kune mirairo yemugadziri yematanho ekugadzirisa matambudziko uye mhinduro.
