PCR Thermal Cycler WD-9402D
Zvinotsanangurwa
| Model | WD-9402D |
| Capacity | 96×0.2ml |
| Tube | 0.2ml chubhu, 8 mitsetse, Half siketi96 matsime ndiro, Hapana siketi 96 matsime ndiro |
| Reaction Volume | 5-100ul |
| Temperature Range | 0-105 ℃ |
| MAX. Ramp Rate | 5℃/s |
| Uniformity | ≤±0.2℃ |
| Kururama | ≤±0.1℃ |
| Display Resolution | 0.1℃ |
| Temperature Control | Block/Tube |
| Ramping Rate Inogadziriswa | 0.01-5℃ |
| Gradient Temp. Range | 30-105 ℃ |
| Gradient Type | Normal Gradient |
| Gradient Kupararira | 1-42 ℃ |
| Hot Lid Temperature | 30-115 ℃ |
| Nhamba Yezvirongwa | 20000 + (USB FLASH) |
| Max. Nhamba yeDanho | 40 |
| Max. Nhamba yeCycle | 200 |
| Nguva Kuwedzera/Kuderera | 1 Sec - 600 Sec |
| Temperature Kuwedzera/Kuderera | 0.1-10.0 ℃ |
| Pause Function | Ehe |
| Auto Data Dziviriro | Ehe |
| Bata pa4 ℃ | Zvachose |
| Touchdown Basa | Ehe |
| Yakareba PCR Basa | Ehe |
| Mutauro | Chirungu |
| Computer Software | Ehe |
| Nharembozha APP | Ehe |
| LCD | 10.1 inch, 1280 × 800 pels |
| Kukurukurirana | USB2.0, WIFI |
| Dimensions | 385mm×270mm×255mm (L×W×H) |
| Kurema | 10kg |
| Magetsi | 100-240VAC , 50/60Hz , 600 W |
Tsanangudzo
Thermal cycler inoshanda nekudzokorodza kupisa nekutonhodza reaction musanganiswa ine DNA kana RNA template, primers, uye nucleotides. Iyo tembiricha yebhasikoro inodzorwa chaizvo kuti iwane inodiwa denaturation, annealing, uye ekuwedzera matanho ePCR maitiro.
Kazhinji, inopisa inotenderera ine chivharo chine matsime akawanda kana machubhu panoiswa reaction musanganiswa, uye tembiricha mutsime rega rega inodzorwa yakazvimirira. Iyo block inodziiswa uye inotonhodzwa uchishandisa Peltier element kana imwe yekudziya uye kutonhora system.
Mazhinji anopisa mabhasikoro ane mushandisi-ane hushamwari interface inobvumira mushandisi kuronga uye kugadzirisa ma paramita eku bhasikoro, senge tembiricha ye annealing, nguva yekuwedzera, uye nhamba yematenderedzwa. Vanogona zvakare kuve nechiratidziro chekutarisa mafambiro ekuita, uye mamwe mamodheru anogona kupa maficha epamberi senge gradient tembiricha yekudzora, akawanda block masisitimu, uye kure kutarisa uye kutonga.
Application
Genome cloning; Asymmetric PCR kugadzirira kwe-single-stranded DNA yeDNA sequencing; Inverse PCR yekugadzika kwenzvimbo dzisingazivikanwe dzeDNA; reverse transcription PCR (RT-PCR). Yekuona danho rekutaura kwemajini mumaseru, uye huwandu hwehutachiona hweRNA uye yakananga cloning yecDNA ine majini chaiwo; kukurumidza kuwedzera kweCDNA magumo; kuonekwa kwekutaura kwejeni; inogona kushandiswa pakuona kwebhakitiriya uye zvirwere zvehutachiona; kuongororwa kwezvirwere zvemajini; kuongororwa kwemamota; tsvakiridzo yezvekurapa senge humbowo hwemuviri, haigone kushandiswa mukurapa kukiriniki yekutsvaga.
Featured
• Kupisa kukuru uye kutonhora kwehuwandu, max. Ramping rate 8 ℃/s;
• Otomatiki restart pashure simba kukundikana. Kana simba radzoreredzwa rinogona kuramba richiita chirongwa chisina kupera;
• Kudzvanya kamwe chete nekukurumidza incubation basa rinogona kusangana nezvinodiwa zvekuyedza senge denaturation, enzyme cutting/enzyme-link uye ELISA;
• Kupisa chivharo tembiricha uye inopisa chivharo basa modhi inogona kusetwa kusangana kwakasiyana kuedza zvinodiwa;
• Inoshandisa tembiricha yebhasikoro-yakananga-hupenyu hurefu Peltier modules;
• Anodized aluminium module ine engineering kusimbiswa, iyo inochengetedza kukurumidza kupisa conduction performance uye ine kukwana kukwana kwekudzivirira;
• Inokurumidza tembiricha ramp rates, ine chiyero chepamusoro che 5 ° C / s, kuchengetedza yakakosha nguva yekuedza;
• Adaptive pressure bar-style thermal cover, iyo inogona kuvharwa zvakasimba nenhanho imwe uye inogona kuenderana nemhando dzakasiyana dzechubhu urefu;
• Pamberi-kumashure-kumashure kugadzirwa kwemhepo, kubvumira michina kuiswa parutivi;
• Inoshandisa Android operating system, inofananidzwa ne 10.1-inch capacitive touch screen, ine graphical menu-style navigation interface, zvichiita kuti kushanda kuve nyore zvakanyanya;
• Yakavakwa-mu 11 yakajairika purogiramu yefaira matemplate, anogona kukurumidza kugadzirisa mafaira anodiwa;
• Kuratidzwa kwechokwadi-nguva yekufambira mberi kwepurogiramu uye nguva yakasara, inotsigira mid-programming yePCR chiridzwa;
• Rimwe-bhatani rinokurumidza kupinza basa, rinosangana nezvinodiwa zvekuedza zvakadai se denaturation, enzyme digestion / ligation, uye ELISA;
• The inopisa chivharo tembiricha uye inopisa chivharo kushanda modhi inogona kusetwa kusangana siyana kuedza zvinodiwa;
• Automatic power-off dziviriro, otomatiki kuita mitsara isina kupera mushure mekunge simba radzoreredzwa, kuve nechokwadi chekushanda kwakachengeteka mukati meiyo amplification process;
• USB interface inotsigira PCR data kuchengetedza / kutora uchishandisa USB drive uye inogonawo kushandisa USB mouse kudzora chiridzwa chePCR;
• Inotsigira zvigadziriso zvesoftware kuburikidza ne USB neLAN;
• Yakavakwa-mukati WIFI module, kubvumira komputa kana mbozhanhare kudzora panguva imwe chete akawanda PCR zviridzwa kuburikidza netiweki kubatana;
• Inotsigira email chiziviso kana chirongwa chekuedza chapera.
FAQ
Mubvunzo: Chii chinonzi thermal cycler?
A: A thermal cycler mudziyo werabhoritari unoshandiswa kukwidziridza DNA kana RNA sequences kuburikidza ne polymerase chain reaction (PCR). Inoshanda nekuchovha bhasikoro kuburikidza nenhevedzano yekushanduka kwetembiricha, ichibvumira kutevedzana kweDNA kukwidziridzwa.
Mubvunzo:Ndezvipi zvikamu zvikuru zve thermal cycler?
A: Izvo zvikamu zvikuru zve thermal cycler zvinosanganisira chivharo chekudziya, thermoelectric cooler, tembiricha sensors, microprocessor, uye control panel.
Mubvunzo: Inoshanda sei bhasikoro rinopisa?
A: A thermal cycler anoshanda nekudziya nekutonhodza DNA samples munhevedzano yetembiricha kutenderera. Nzira yebhasikoro inosanganisira denaturation, annealing, uye nhanho dzekuwedzera, imwe neimwe ine tembiricha yakatarwa uye nguva. Aya madenderedzwa anobvumira kutevedzana kweDNA kuti kukwidziridzwe kuburikidza ne polymerase chain reaction (PCR).
Mubvunzo: Ndezvipi zvakakosha zvekufunga nezvazvo pakusarudza thermal cycler? A: Zvimwe zvinhu zvakakosha zvekufunga nezvazvo pakusarudza chitenderedzwa chekupisa zvinosanganisira huwandu hwematsime kana machubhu ekuita, tembiricha yemhando uye kumhanya kwerampu, iko kurongeka uye kufanana kwekudzora tembiricha, uye mushandisi interface uye software kugona.
Mubvunzo: Unochengetedza sei bhasikoro rinopisa?
A: Kuti uchengetedze bhaisikopo rinopisa, zvakakosha kugara uchichenesa chivharo chekudziya uye machubhu ekuita, tarisa kusakara uye kubvaruka pazvikamu, uye kugadzirisa tembiricha sensors kuti ive nechokwadi chekudzora tembiricha. Izvo zvakakoshawo kutevedzera mirairo yemugadziri wekugara uchigadzirisa uye kugadzirisa.
Mubvunzo: Ndeapi mamwe matanho akajairika ekugadzirisa dambudziko kune anopisa cycler?
A: Mamwe matanho akajairika ekugadzirisa dambudziko remudhudhudhu anopisa anosanganisira kutarisa zvinhu zvakasununguka kana zvakakanganisika, kuona tembiricha yakakodzera uye nguva, uye kuyedza machubhu ekuita kana ndiro kuti ane tsvina kana kukuvara. Izvo zvakakoshawo kutarisa kune mirairo yemugadziri yematanho ekugadzirisa matambudziko uye mhinduro.
