Chii chinonzi thermal cycling process muPCR?

Polymerase Chain Reaction (PCR) inzira yemamorekuru biology inoshandiswa kukudza zvimedu zveDNA. Inogona kutorwa semhando yakakosha yeDNA replication kunze kwechinhu chipenyu. Chinhu chikuru chePCR kugona kwayo kuwedzera zvakanyanya kuteedzera huwandu hweDNA.

Mhedziso yePolymerase Chain Reaction Cycle

Mufananidzo kubva kuBioninja

Chii chinonzi thermal cycling process muPCR?

PCR ine matanho matatu ekutanga:

1. Denaturation of Template DNA: The template DNA inopisa kusvika ku93 ° C kwenguva yakati, zvichiita kuti DNA ine tambo mbiri iparadzanise kuita tambo imwe chete, ichigadzirira kutanga kusungirirwa mune imwe nzira inotevera.

2. Anealing of Template DNA and Primers: After denaturation, tembiricha inodzikiswa kusvika pa55°C, zvichibvumira maprimers kuti abatanidze kune anowirirana sequences pane imwe-stranded template DNA.

3. Kuwedzerwa kwePrimers: Pa 72 ° C, DNA template-primer complex inowedzera nerubatsiro rweDNA polymerase (kufanana neTaq DNA polymerase), uchishandisa dNTPs se substrates kuti iite mutsara mutsva wekubatanidza zvichienderana neDNA template.

Beijing LIUYI PCR Machine

Nekudzokorodza denaturation, annealing, uye maitiro ekuwedzera, mamwe "semi-Conservative replication tambo" anogadzirwa, ayo anogona kushanda sematemplate ekutenderera anotevera. Kutenderera kwega kwega kunotora maminetsi maviri kusvika ku4, zvichiita kuti jini rinonangwa riwedzere mamirioni enguva mukati memaawa maviri kusvika matatu.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ine hunyanzvi mukugadzira zviridzwa zve electrophoresis kweanopfuura makore makumi mashanu nechikwata chedu chehunyanzvi chehunyanzvi uye R&D centre. Isu tine yakavimbika uye yakakwana yekugadzira mutsara kubva padhizaini kusvika pakuongorora, uye imba yekuchengetera, pamwe nerutsigiro rwekushambadzira. Zvigadzirwa zvedu zvikuru ndeElectrophoresis Cell (tangi/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Isu tinopawo zvishandiso zvelab sePCR instrument, vortex mixer uye centrifuge ye laboratori.

Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu. Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.

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