Nhanho-ne-Nhanho Nhungamiro Yekugadzirira Agarose Gel ye Electrophoresis

Iwe une chero matambudziko mukugadzira agarose gel?Regai titeverenyanzvi yedu yerabhu mukugadzira gel.

Kugadzirira kweagarose gel kunosanganisira matanho anotevera:

Kuyera Agarose Powder

Weresa huwandu hunodiwa hwehupfu hweagarose maererano neinoda fungidziro yekuedza kwako. Yakajairika agarose concentrations kubva ku0.5% kusvika 3%. Kukwirira kwepamusoro kunoshandiswa kupatsanura madiki mamorekuru eDNA, nepo mashoma ari emamorekuru makuru.

Kugadzirira Buffer Solution

Wedzera agarose powder kune yakakodzera electrophoresis buffer, yakadai se1 × TAE kana 1 × TBE. Huwandu hwebuffer hunofanirwa kuenderana nehuwandu hwejeli inodiwa pakuedza kwako.

Kubvisa Agarose

Pisa musanganiswa weagarose uye buffer kusvikira agarose yanyungudika zvachose. Izvi zvinogona kuitwa ne microwave kana hot plate. Gadzirisa mushonga wacho nguva nenguva kuti urege kufashaira. Iyo agarose solution inofanira kujeka pasina chero zvidimbu zvinooneka.

Kutonhodza iyo Agarose Solution

Bvumira mushonga weagarose inopisa kuti utonhore kusvika pa50-60°C. Gadzirisa mhinduro panguva yekutonhora kudzivirira kusimba kusati kwasvika.

Kuwedzera Nucleic Acid Stain (Sarudzo)

Kana iwe uchida kuona DNA kana RNA mujeri, unogona kuwedzera nucleic acid stain, yakadai seGelRed kana ethidium bromide, panguva ino. Paunenge uchibata mavara aya, pfeka magirovhosi uye chenjerera, sezvo anogona kuve ane chepfu.

Kukanda Gel

Dururirai yakatonhodzwa agarose mhinduro mune yakagadzirirwa electrophoresis gel mold. Isa muzinga kuti ugadzire matsime emuenzaniso, kuve nechokwadi kuti muzinga wakachengeteka uye mhinduro yacho yakagoverwa zvakaenzana muforoma.

 Gel Solidification

Bvumira iyo gel kuti iome pakupisa kwekamuri, izvo zvinowanzotora 20-30 maminetsi zvichienderana nekudzika uye ukobvu hwegel.

Rkufambisa Muzinga

Kana gel ranyatsosimba, nyatsobvisa muzinga kuratidza matsime emuenzaniso. Isa gel pamwe chete ne mold mukamuri ye electrophoresis uye uifukidze nehuwandu hwakakodzera hwe electrophoresis buffer, kuve nechokwadi kuti gel rakazara pasi.

Kugadzirira Electrophoresis

Mushure mekunge gel yagadzirira, takura masampuli ako mumatsime, uye enderera mberi nekuedza kwe electrophoresis.

Kana iwe uine chero nyaya nekugadzirira gel, inzwa wakasununguka kutibata nesu. Tine nyanzvi dzema lab tekinoroji dziripo dzekutaurirana newe.

Isu tinofara kugovera dzimwe nhau dzakanaka: yedu yakakurumbira DYCP-31DN horizontal electrophoresis tank parizvino iri kusimudzirwa., taura nesu kuti uwane rumwe ruzivo izvozvi!

DYCP-31DN horizontal electrophoresis tank

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ine hunyanzvi mukugadzira zviridzwa zve electrophoresis kweanopfuura makore makumi mashanu nechikwata chedu chehunyanzvi chehunyanzvi uye R&D centre. Isu tine yakavimbika uye yakakwana yekugadzira mutsara kubva padhizaini kusvika pakuongorora, uye imba yekuchengetera, pamwe nerutsigiro rwekushambadzira. Zvigadzirwa zvedu zvikuru ndeElectrophoresis Cell (tangi/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Isu tinopawo zvishandiso zvelab sePCR instrument, vortex mixer uye centrifuge ye laboratori.

Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu. Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.

Ndokumbira utarise kodhi yeQR yekuwedzera paWhatsapp kana WeChat.