Protocol for Pre-cast Gel Protein Electrophoresis Experiment

Kugadzirira Kuedza

Tarisa Zvishandiso: Ita shuwa kuti protein electrophoresis chamber, magetsi ekupa, uye kutamisa system zviri kushanda.TinopaDYCZ-24DN yeprotein electrophoresis,DYCZ-40D yekufambisa system, uyeDYY-6C yemagetsi.

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Kugadzirira Sample: Gadzirira sampuli dzako zvinoenderana neyakaedzwa dhizaini. Rapa mapuroteni samples nezvinoderedza uye maprotease kana zvichidikanwa.

Gadzirira Electrophoresis Buffer: Tevedzera mirairo yakapihwa nepre-cast gel kuti ugadzirire electrophoresis buffer panguva yakakodzera.

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Kubata iyo Pre-cast Gel:

Bvisa Pre-cast Gel: Nyatsovhura kurongedza uye bvisa pre-cast gel kubva mumudziyo wayo, uchichenjerera kuti usakuvadza gel matrix.

Sample Loading: Takura sampuli dzako dzakagadzirirwa mumatsime emuenzaniso wegel uchishandisa micropipette kana mamwe maturusi akakodzera. Teerera kurodha yekurodha uye vhoriyamu yemuenzaniso wega wega.

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Set Electrophoresis Conditions: Isa iyo electrophoresis mamiriro, kusanganisira ikozvino intensity, voltage, uye nguva. Ita shuwa kuti unosarudza mamiriro akakodzera kupatsanurwa kwakaringana.

Kushandisa Electrophoresis

Tanga Electrophoresis: Isa gel mukamuri ye electrophoresis, batanidza magetsi, uye tanga electrophoresis. Tarisa maitiro kuti uone kugadzikana kwezvino.

Complete Electrophoresis: Misa iyo electrophoresis kana masampuli atamira kunzvimbo dzinodiwa. Dzivisa kumhanyisa electrophoresis kwenguva yakareba kuti udzivise masampuli kubva pakupera kwegel.

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Kuendesa Mapuroteni

Gadzirira Transfer System: Tora gel ndiro kunze kwekamuri uye ugadzirire kutamisa mapuroteni. Izvi zvinogona kusanganisira kucheka membrane uye kugadzirira iyo yekufambisa buffer.

Assemble Transfer Setup: Unganidza iyo protein yekufambisa setup zvinoenderana nemirairo inopihwa neyekutamisa system. Chenjerera kuhurongwa hwegungano uye kuisa mamiriro.

Run Protein Transfer: Tanga iyo protein yekufambisa maitiro uye tarisa mafambiro ayo. Ita shuwa kuti nguva yekufambisa uye mamiriro anoenderana nezvinodiwa zvekuyedza kwako.

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Post-Transfer Processing:

Membrane Kubata: Gadzirisa iyo yakatamiswa membrane sezvinodiwa, izvo zvinogona kusanganisira kusvibisa, immunoblotting, kana mamwe maitiro ekutamisa-shure zvichienderana nekuedza zvinodiwa.

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Mhedzisiro Ongororo: Ongorora zvawanikwa zvichibva pane yako yekuyedza dhizaini uye matanho ekugadzirisa. Dudzira zvakawanikwa uye gadzira machati akakodzera kana magirafu.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ine hunyanzvi mukugadzira zviridzwa zve electrophoresis kweanopfuura makore makumi mashanu nechikwata chedu chehunyanzvi chehunyanzvi uye R&D centre. Isu tine yakavimbika uye yakakwana yekugadzira mutsara kubva padhizaini kusvika pakuongorora, uye imba yekuchengetera, pamwe nerutsigiro rwekushambadzira. Zvigadzirwa zvedu zvikuru ndeElectrophoresis Cell (tangi/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc.

Isu tave kutsvaga vanobatika, vese OEM electrophoresis tank uye vanogovera vanogamuchirwa.

Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu. Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.

Ndokumbira utarise kodhi yeQR yekuwedzera paWhatsapp kana WeChat.

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Nguva yekutumira: Zvita-15-2023