Protein Electrophoresis Common Issues

Protein electrophoresis band issues zvinoreva matambudziko kana kusarongeka kunogona kumuka panguva yekuparadzanisa mapuroteni zvichienderana nechaji yavo yemagetsi vachishandisa electrophoresis. Idzi nyaya dzinogona kusanganisira kutaridzika kwemabhendi asingatarisirwi kana abnormal, kusagadziriswa zvakanaka, kuzora, kana kukanganisa kweprotein band, pakati pezvimwe. Isu tinopfupisa nyaya dzinoverengeka dzakajairika kuti vatengi vedu vatarise kuti vawane zvikonzero uye kuwana mhinduro chaidzo uye nekuvandudza kunaka kwekuparadzaniswa kweprotein.

Smileband- bhendi pateni inotenderera ichikwira kumusoro kumativi ese egel

Chikonzero
1. Pakati pejeri rinopisa kupfuura chero magumo
2. Mamiriro emagetsi akawandisa

Solution
① Buffer isina kusanganiswa zvakanaka kana buffer mukamuri yepamusoro yakanyura zvakanyanya. Gadzirisa buffer, kuve nechokwadi kusanganisa kwakaringana, kunyanya kana uchibvisa 5x kana 10x stock.
② Deresa kugadzika kwemagetsi kubva pa200 V kusvika pa150 V kana kuzadza kamuri yepasi kusvika mukati me1 cm pamusoro peplate pfupi.

Vertical streaking yeprotein

Chikonzero
1. Muenzaniso wakawandisa
2. Sample precipitation Solution

Solution
① Dilute sampuli, sarudza bvisa mapuroteni akawanda mumuenzaniso, kana kuderedza voltage inosvika 25% kuderedza kutenderera.
② Centrifuge sampu isati yawedzerwa SDS sampuli buffer, kana kuderedza% T yegel.
③ Chiyero cheSDS kuprotein inofanira kukwana kuti ipfeke imwe neimwe protein molecule neSDS, kazhinji 1.4: 1. Inogona kuda yakawanda SDS kune mamwe membrane protein samples

Buyenehorizontallykupararira

Chikonzero
1. Kupararira kwematsime vasati vashandura ikozvino
2. Ionic simba remuenzaniso yakaderera pane iyo gel

Solution
① Deredza nguva pakati pemuenzaniso application uye kubatidza simba rekutanga
② Shandisa imwechete buffer mumuenzaniso semujeri kana gel yakaturikidzana

Mabhendi eprotein akashatiswa kana kutsveyamiswa

Chikonzero
1. Yakashata polymerization yakatenderedza matsime
2. Munyu mumuenzaniso
3. Kusaenzana kwegel interface

Solution
① Degas kurongedza gel mhinduro zvachose isati yakanda; wedzera ammonium persulfate uye TEMED concentrations ne25%, yekurongedza gel kana yakaderera% T, siya APS yakafanana uye kaviri iyo TEMED yekusungwa.
② Bvisa munyu ne dialysis, desalting;
③ Deredza mwero wepolymerization. Fukidza gels zvakanyatsonaka.

Beijing Liuyi Biotechnology Company Ltd inogadzira huwandu hwezvigadzirwa zve electrophoresis zvinogona kubatsira kugadzirisa nyaya dzatingangosangana nadzo mukuyedza kwedu kwe electrophoresis.

Beijing Liuyi Biotechnology Company Ltd yakavambwa muna 1970, yaimbozivikanwa seBeijing Liuyi Instrument Factory, inogadzira michina yemurabhoritari uye zviridzwa zvesainzi zvakavakirwa muBeijing, China. Iine makore anopfuura makumi mashanu ruzivo mukugadzira nekugadzira zvigadzirwa zve electrophoresis uye tsvakiridzo yesainzi yehupenyu, inova mupi anotungamira wezviridzwa zverabhoritari uye mhinduro muChina.Zvigadzirwa zvekambani zvinosanganisira zvakasiyana-siyana zvezviridzwa zverabhoritari, zvinosanganisira horizontal nucleic acid electrophoresis tank, vertical protein. electrophoresis tank/unit, black-box type UV analyzer, Gel Document Tracking Imaging Analyzer, uye electrophoresis magetsi. Zvigadzirwa izvi zvinoshandiswa zvakanyanya mumasangano ekutsvagisa, mayunivhesiti, uye akasiyana maindasitiri, akadai semishonga, biotechnology, uye yekuongorora kwezvakatipoteredza.Kambani iyi ISO9001 & ISO13485 yakasimbiswa kambani uye ine CE zvitupa.

Panezvakasiyana-siyana zveverticalelectrophoresis matangi nokudaprotein electrophoresiskuongororwa uye kuzivikanwa kweprotein samples ne polyacrylamide gel electrophoresis,uyezvakare yekuyera masamples 'molecular uremu, kuchenesa masampuli uye kugadzirira masampuli.Zvigadzirwa izvi zvese zvinogamuchirwa mukatimusika wemunyika nemhiri kwemakungwa.

Iyo electrophoresis simba rekupa chinhu chakakosha cheelectrophoresis system, kupa chitubu chakatsiga uye chakanyatsojeka chemagetsi ekufambisa nzira yekuparadzanisa.Itkazhinji inoburitsa voltage inogara iripo kana inogara iripo kune electrophoresis system, zvichienderana neiyo chaiyo yekuyedza protocol. Iyo zvakare inobvumira mushandisi kugadzirisa voltage kana yazvino kubuda, pamwe nemamwe maparamita akadai senguva uye tembiricha, kukwidziridza mamiriro ekuparadzanisa kune kumwe kuedza.

Fkana kutarisa gel, unogona kusarudza UV Transilluminator WD-9403 yakatevedzana yakagadzirwa neBeijing Liuyi Biotechnology.A UV transilluminator chishandiso chemurabhoritari chinoshandiswa kuona uye kuongorora DNA, RNA, uye mapuroteni samples. Inoshanda nekuvhenekera masampuli neUV mwenje, izvo zvinoita kuti masampuli aite fluoresce uye aonekwe. Kune akati wandei modhi yeUV transilluminatorzvakapiwa nokuda kwenyu nesu. WD-9403A inonyanya kucherechedza protein electrophoresis, uye WD-9403F inoshandiswa kuona DNA uye protein electrophoresis.

Aya akatevedzana ezvigadzirwa anogona kushanda kubva pakukandira gel kusvika pakuona gel zvinoenderana nezvaunoda kuedza.Tizivise zvaunoda, zvose OEM, ODM uye vaparidzi vanogamuchirwa.We achaedza zvatinokwanisa kuti tikupei zvigadzirwa zvedu nemasevhisi.

Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu. Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.