Electrophoresis inzira inoshandiswa kupatsanura mabiomolecules zvichienderana nekukura kwawo uye kuchaja uchishandisa munda wemagetsi. Inoshandiswa zvakanyanya mubiology sainzi nekuda kwezvinangwa zvakasiyana, kubva pakuongorora kweDNA kusvika kucheneswa kweprotein. Pano, tinoongorora nheyo ye electrophoresis uye maitiro ayo akasiyana-siyana.
Nheyo yeElectrophoresis
Electrophoresis inotsamira pakufamba kwezvimedu zvinochajiswa mumunda wemagetsi. Iyo yekutanga setup inosanganisira kuisa sampuli (ine inochajiwa biomolecules) pane gel kana mune mhinduro, uye nekuisa yemagetsi ikozvino. Iwo mabiomolecules anofamba nepakati nepakati pamitengo yakasiyana zvichienderana nechaji uye saizi, zvichikonzera kupatsanurwa.
Mhando dzeElectrophoresis
1. Gel Electrophoresis
Agarose Gel Electrophoresis: Inoparadzanisa DNA neRNA zvimedu zvichienderana nehukuru.
Polyacrylamide Gel Electrophoresis (PEJI): Inogadzirisa mapuroteni zvichienderana nehukuru uye mutero.
2. Capillary Electrophoresis
Inoshandisa yakamanikana capillaries kupatsanurwa, zvichibvumira kuongororwa nekukurumidza kweDNA, RNA, uye mapuroteni.
Zvishandiso muBiological Sayenzi
1. Kuongorora kweDNA
Genotyping: Inozivisa kusiyana kwemajini (semuenzaniso, SNPs) inobatanidza nezvirwere.
DNA Sequencing: Inotarisa kurongeka kwemanucleotides muDNA molecule.
DNA Fragment Analysis: Size DNA zvimedu zvekushandisa mune molecular biology.
2. RNA Analysis
RNA Electrophoresis: Inopatsanura maRNA mamorekuru ekuongorora gene expression uye RNA kuperera.
3. Mapuroteni Analysis
SDS-PEJI (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis): Inoparadzanisa mapuroteni zvichienderana nehukuru.
2D Electrophoresis: Inobatanidza isoelectric inotarisa uye SDS-PAGE kuparadzanisa mapuroteni anobva pane isoelectric point uye saizi.
4. Kucheneswa
Preparative Electrophoresis: Inochenesa biomolecules (semuenzaniso, mapuroteni) zvichienderana nechaji uye saizi.
5. Clinical Applications
Hemoglobin Electrophoresis: Inoongorora hemoglobinopathies (semuenzaniso, sickle cell disease).
Serum Protein Electrophoresis: Inozivisa kusaenzana muSerum mapuroteni.
6. Forensic Applications
DNA Profileing: Inofananidzira DNA samples yekuferefeta kuferefeta.
Zvakanakira Electrophoresis
High Resolution: Inoparadzanisa biomolecules zvichienderana nehukuru uye kubhadharisa nehupamhi hwepamusoro.
Versatility: Inoshanda kune DNA, RNA, mapuroteni, uye mamwe anochajwa biomolecules.
Quantitative Analysis: Inoyera huwandu hwema biomolecules zvichienderana nekusimba kwebhendi.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ine hunyanzvi mukugadzira zviridzwa zve electrophoresis kweanopfuura makore makumi mashanu nechikwata chedu chehunyanzvi chehunyanzvi uye R&D centre. Isu tine yakavimbika uye yakakwana yekugadzira mutsara kubva padhizaini kusvika pakuongorora, uye imba yekuchengetera, pamwe nerutsigiro rwekushambadzira. Zvigadzirwa zvedu zvikuru ndeElectrophoresis Cell (tangi/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Isu tinopawo zvishandiso zvelab sePCR instrument, vortex mixer uye centrifuge ye laboratori.
Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu. Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.
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Nguva yekutumira: May-28-2024