Kugadzirira kweAgarose Gel ye Electrophoresis
Cherechedza: Gara uchipfeka magirovhosi anoraswa!
Nhanho-ne-Nhanho Mirayiridzo
Kuyera Agarose Powder:use bepa rekuyera uye chiyero chemagetsi chekuyera 0.3g yeagarose powder (yakavakirwa pane 30ml system).
Kugadzirira TBST Buffer:pgadzirisa 30ml ye1x TBST buffer mu100ml Erlenmeyer flask.
Kubvisa Agarose Powder:pyedu agarose hupfu muTBST buffer uye zunza zvakanaka.Vaise mukatikune microwave uye kupisa (kazhinji kwemasekonzi makumi mashanu, akafukidzwa nealuminium foil) kusvikira yapera zvachose.
Kutonhora uye Kuwedzera Nuclease:use magirovhosi kuti ubvise musanganiswa kubva mumicrowave worega utonhore zvishoma mumvura inotonhora kusvika yadziya (inenge 60°C). Wedzera 2µl ye nuclease (Eb inotsiva) uchizunza kusanganisa zvakakwana.
Kugadzirira Gel Mold:
- Ckonda nekuomesa iyogel tray uye gel casting devicetangi ye electrophoresis.
- Place geltraymutangi remukati wopinza muzinga panzvimbo yakamira.
- Sanganisa mushonga weagarose gel, wakatonhodzwa kusvika pa65°C, wonyatsoudururira pairigel traymugel casting device, achiiparadzira zvishoma nezvishoma kusvikira yaita kunyange gel layer pane ndiro yegirazi.
- Bvumira kuti igare pakushambidzika kwekamuri kusvikira gel raoma zvachose.
- Bvisa muzinga wakamira zvinyoro nyoro wobvisa tepi.
- Isa geltraymu electrophoresis tank.
Zvakakosha: Ita shuwa kuti hapana mabhuru emhepo panzvimbo yemazino emizinga. Pamusoro pemvura inofanira kuva yakatsetseka pasina ripples.
Kumhanya Gel
Loading the Gel
Mushure mokunge gel raoma, riise mukati me electrophoresis tank uye udururire electrophoresis buffer kusvikira gel yanyura.
Kugadzirira Samples
- Tora chiratidzo uye kurodha buffer kubva mufiriji.
- Wedzera 6µl yekurodha buffer kumasampuli uye sanganisa zvakanaka.
- Uchishandisa micropipette, zvishoma nezvishoma takura sampuli mumatsime makuru ejeri (chenjerera kuti urege kubaya gel uye usaparadzira vhoriyamu yose kudzivirira mabhuru emhepo).
- Isa chiratidzo mune chero matsime madiki (rangarira chinzvimbo chayo).
Kutanga Electrophoresis
- Ivhara tangi ye electrophoresis uye tanga electrophoresis pakarepo mushure mekutakura gel.
- Isa iyo voltage kune 60-100V. Iwo masampuli achatama kubva kune yakaipa electrode (dema) kuenda kune yakanaka electrode (tsvuku).
- Higher voltage inopfupisa iyo inoshanda yekuparadzanisa huwandu hweiyo agarose gel.
- Misa electrophoresis apo bromophenol dhayi yebhuruu inosvika anenge 1cm kubva kumucheto wepasi peplate yegel.
Kucherechedza Migumisiro
Mushure mekuparadzanisa mabhanhire, rega electrophoresis, bvisa gel, uye uone zvakananga uye uiite mufananidzo.
Shandisa gel imaging system kutora uye kutarisa mabhendi (tarisa kana paine mabhendi emaka kana samples).
Mushure mekutora mepu yako yebhendi yegel, tsvaga chiratidzo. Zvichienderana nemucherechedzo, unogona kuona mabhendi anonangwa!
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) yakagadzirwa zvigadzirwa zve electrophoresis kwemakore anopfuura makumi mashanu. Isu tinopa yedu horizontal electrophoresis masisitimu pano kuitiraagarose gelelectrophoresis.Sarudzahorizontal electrophoresis systemskubva kuBeijing liuyi kune yako gel electrophoresis kuyedza kubatsira tsvagiridzo yako muindasitiri yebiotechnology.
Isu tinopa akasiyana mamodheru ehorizontal electrophoresis matangi ekukanda uye kumhanya madikiagarosegels kusvika kuhombeagarosegels
Sarudzo Yekutungamira yeHorizontal Immersed Gel Electrophoresis Unit
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ine hunyanzvi mukugadzira zviridzwa zve electrophoresis kweanopfuura makore makumi mashanu nechikwata chedu chehunyanzvi chehunyanzvi uye R&D centre. Isu tine yakavimbika uye yakakwana yekugadzira mutsara kubva padhizaini kusvika pakuongorora, uye imba yekuchengetera, pamwe nerutsigiro rwekushambadzira. Zvigadzirwa zvedu zvikuru ndeElectrophoresis Cell (tangi/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Isu tinopawo zvishandiso zvelab sePCR instrument, vortex mixer uye centrifuge ye laboratori.
Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu. Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.
Ndokumbira utarise kodhi yeQR yekuwedzera paWhatsapp kana WeChat.
Nguva yekutumira: Aug-09-2024