Polymerase Chain Reaction (PCR) uye Gel Electrophoresis: Maitiro Akakosha muMolecular Biology.

Mundima inogara ichishanduka yemamolekyuli biology, Polymerase Chain Reaction (PCR) uye Gel Electrophoresis zvakabuda senzira dzepakona dzinofambisa kudzidza uye kushandura DNA. Idzi nzira hadzina kungobatanidzwa chete mukutsvagisa asiwo dzine mashandisirwo akapararira mukuongorora, forensic sainzi, uye biotechnology.

Mufananidzo kubva webhusaiti biology4alevel

PCR inzira yekuchinja yakagadziridzwa naKary Mullis muna 1983, iyo inobvumira masayendisiti kukwidziridza chimwe chikamu cheDNA exponentially. Iyo nzira inotanga ne denaturation yeDNA, apo DNA ine tambo mbiri inopisa kusvika ku94 ° C, zvichiita kuti iparadzanise kuita tambo mbiri imwe chete. Izvi zvinoteverwa neannealing, apo maprimers-short sequences of nucleotides-anosungirirana kune anokwana kutevedzana paDNA ine tambo imwe patembiricha yakadzikira (kazhinji inotenderera 55°C). Pakupedzisira, chikamu chekuwedzera chinoitika pa 72 ° C, apo enzyme DNA polymerase inogadzirisa tambo itsva yeDNA nekuwedzera nucleotides kune zvinyorwa. Kutenderera uku kunodzokororwa 20-40 nguva, zvichitungamira kumamiriyoni emakopi echinangwa cheDNA kutevedzana.

Beijing LIUYI PCR Machine

Kana DNA ichinge yawedzerwa, Gel Electrophoresis inoshandiswa kuparadzanisa uye kuongorora zvigadzirwa zvePCR. Iyi nzira inosanganisira kutama kwezvidimbu zveDNA kuburikidza neagarose gel matrix pasi pesimba remunda wemagetsi. Mamorekuru eDNA anochajwa zvisina kunaka nekuda kwemusana wephosphate, uye anotama akananga kune yakanaka electrode. Iyo gel inoshanda sesefa, ichibvumira zvidimbu zvidiki zveDNA kuti zvifambe nekukurumidza kupfuura zvakakura. Nekuda kweizvozvo, zvidimbu zveDNA zvinopatsanurwa zvichienderana nehukuru hwazvo, nemabhandi akasiyana anoonekwa pasi pechiedza cheultraviolet mushure mekusvibiswa nedhayi senge ethidium bromide.

Beijing LIUYIGel Electrophoresis Zvigadzirwa

Iko kusanganiswa kwePCR uye Gel Electrophoresis kune simba mune zvakawanda zvekushandisa. Mukuongorora kwekurapa, PCR inoshandiswa kuona kuvepo kwezvirwere, genetic mutations, kana kutevedzana kweDNA kwakanangana nechirwere. Gel Electrophoresis inobva yabvumira kuonekwa uye kusimbiswa kwezvimedu zveDNA zvakasimudzwa. Musainzi yezvemberi, matekiniki aya akakosha pakudhindisa zvigunwe zveDNA, kwaanobatsira kuenzanisa masampula eDNA kubva munzvimbo dzematsotsi neaya evanofungira.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ine hunyanzvi mukugadzira zviridzwa zve electrophoresis kweanopfuura makore makumi mashanu nechikwata chedu chehunyanzvi chehunyanzvi uye R&D centre. Isu tine yakavimbika uye yakakwana yekugadzira mutsara kubva padhizaini kusvika pakuongorora, uye imba yekuchengetera, pamwe nerutsigiro rwekushambadzira. Zvigadzirwa zvedu zvikuru ndeElectrophoresis Cell (tangi/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Isu tinopawo zvishandiso zvelab sePCR instrument, vortex mixer uye centrifuge ye laboratori.

Beijing LIUYIGel Electrophoresis Zvigadzirwa

Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu. Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.

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