Polyacrylamide Gel Electrophoresis

Polyacrylamide inowanzo shandiswa mumamorekuru biology application senge medium ye electrophoresis yemaprotein uye nucleic acids muhunyanzvi unozivikanwa sePEJI. Iyo imhando ye zone electrophoresis nzira ne synthetics gel inonzi polyacrylamide senge inotsigira svikiro. Yakavakwa naS.Raymond naL.Weintraub muna 1959, ichibva yasimudzirwa nekuvandudzwa naL.Ornstein naBJ Davis. Iyi nzira yakashandiswa zvakanyanya mushure meimwe tsananguro uye kugadziridzwa navo mudzidziso uye nzira yekuyedza muna 1964.
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Usati washandisa polyacrylamide gel electrophoresis, vanhu vanonyanya kushandisa bepa electrophoresis yezone EP. Asi bepa rinoita sesvikiro, ringove nebasa reanti-convection, hapana imwe mhedzisiro yakanaka. Nepo iyo polyacrylamide gel isina basa reanti-convection chete asi zvakare inogona kubatanidzwa mukuparadzanisa kuenderera mberi nesimba. Nekuti polyacrylamide gel imhando yemambure chimiro, inova polymerization uye muchinjika-inobatanidza musanganiswa weacrylamide (Acr) uye N, N-methylenebis (acrylamide). Acrylamide inonzi monomer, nepo N,N-methylenebis ichinzi comonomer kana crosslinker. Kugadzira gel inzira yemakemikari polymerization. Iyo pore saizi yegel inogona kudzorwa, saka iyo gel inogona kugadzirwa neakasiyana-akabatana madhigirii. Kana saizi yepore iri kusvika paavhareji radius yemorekuru wesample, kuramba kwemorekuru kuti upinde nepapore yegel kuchava nehukama hwepedyo nehukuru uye chimiro chemorekuru panguva ye electrophoresis. Saka inopa chinoshanduka chekuparadzanisa chinhu chekuparadzanisa izvo zvinhu zvine mambure machaji akafanana.

Polyacrylamide-Gel-Electrophoresis-PEJI

Pane nzira mbiri dzakajairika dzinoshandiswa polyacrylamide gel electrophoresis, imwe disc electrophoresis, uye imwe yacho slab electrophoresis. Iyo slab electrophoresis yakagadzirirwa kupatsanura mapuroteni neDNA, uye kazhinji, kune marudzi maviri e-slab electrohporesis, ayo ari horizontal electrophoresis tank uye vertical electrophoresis tank. Kune mapuroteni, vanhu vanoshandisa horizontal electrophoresis tank yeIFF uye immunoelectrophoresis, zvikasadaro, vanhu vanoshandisa vertical electrophoresis matangi emapuroteni.

Beijing Liuyi Biotechnology ine marudzi akasiyana eslab electrophoresis matangi ePEJI, kunze kwekuongorora uye kuzivikanwa kweprotein samples nepolyacrylamide gel electrophoresis, inogona zvakare kushandiswa kuyera masamples 'mamorekuru huremu, kuchenesa masampuli uye kugadzirira masampuli.

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Tora muenzanisoDYCZ-23Asemuenzaniso, inova yakajairika vertical electrophoresis tank yeLab. Shandisa magirazi maviri egirazi kugadzira kamuri yegel kugadzira gel, uye woombera magirazi egirazi zvakasimba kudzivirira kubuda kwegel. Ukobvu hwejeri hunoenderana nehupamhi hwe spacer. Kazhinji, ukobvu ndeye 1.5mm yekuburitsa kupisa kurinani, uyeDYCZ-23Ainopawo 1.0mm ukobvu spacers yekukanda 1.0mm gel. Kunze kwe electrophoresis tangi, kuitira kuti uite electrophoresis, magetsi anodiwa zvakare. Beijing Liuyi Biotechnology inopa dzakasiyana siyanaelectrophoresis simba rekupa. Kubva pane yakakwira voltage kusvika yakaderera voltage, unosarudza modhi zvinoenderana nekushandisa.

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Iyo Beijing Liuyi mhando ine anopfuura makore makumi mashanu nhoroondo muChina uye kambani inogona kupa yakagadzikana uye yemhando yepamusoro zvigadzirwa pasi rese. Kupfuura makore ebudiriro, yakakodzera sarudzo yako!

Isu tave kutsvaga vanobatika, vese OEM electrophoresis tank uye vanogovera vanogamuchirwa.

Kuti uwane rumwe ruzivo nezvedu, ndapota taura nesu neemail[email yakachengetedzwa]kana[email yakachengetedzwa].


Nguva yekutumira: Oct-12-2022