Nhanganyaya
Gel electrophoresis inzira yakakosha mumolecular biology, inoshandiswa zvakanyanya kupatsanura mapuroteni, nucleic acids, uye mamwe macromolecules. Kudzora kwakaringana kwevhoriyamu yemuenzaniso, voltage, uye nguva ye electrophoresis kwakakosha kuti uwane mibairo yakarurama uye inoberekazve.Wedu lab watinoshanda naye anopamaitiro akanakisa ekugadzirisa aya paramita panguva yeSDS-PAGE gel electrophoresis.
Beijing Liuyi Biotechnology gel electrophoresis zvigadzirwa
Sample Vhoriyamu: Kuvimbisa Kuenderana
Paunenge uchiita SDS-PAGE electrophoresis, vhoriyamu yemuenzaniso chinhu chakakosha chinogona kukanganisa zvakanyanya kugadziriswa kwemhedzisiro yako. Zvinowanzokurudzirwa kurodha 10 µL yeprotein yakazara patsime. Kuve nechokwadi chekuenderana uye kudzivirira kuparadzaniswa kwemuenzaniso pakati pematsime ari padyo, zvakakosha kurodha yakaenzana vhoriyamu ye1x yekurodha buffer mumatsime asina chinhu. Kungwarira uku kunobatsira kudzivirira kupararira kwemasampuli mumigwagwa yepedyo, izvo zvinogona kuitika kana tsime rikasiiwa risina chinhu.
Usati warodha sampuli dzako, gara uchitanga nekuwedzera molecular weight marker kune rimwe tsime. Izvi zvinobvumira kuzivikanwa nyore kwemapuroteni saizi mushure me electrophoresis.
Voltage Kudzora: Kuenzanisa Kumhanya uye Kugadzirisa
Iyo voltage inoshandiswa panguva ye electrophoresis inobata zvakananga zvose kumhanya uko masampuli anofamba kuburikidza nejeri uye kugadzirisa kwekuparadzanisa. Kune SDS-PEJI, zvinokurudzirwa kutanga nemhepo yakaderera ye80V. Iyi yekutanga yakaderera voltage inobvumira masampula kuti afambe zvishoma nezvishoma uye zvakaenzana, achiaisa mubhendi rakapinza sezvaanopinda mujeri rekuparadzanisa.
Kana masampuli anyatsopinda mujero rekuparadzanisa, magetsi anogona kuwedzerwa kusvika ku120V. Iyi voltage yakakwira inomhanyisa kutama, kuve nechokwadi chekuti mapuroteni akapatsanurwa zvakanaka zvichienderana nehuremu hwawo. Zvakakosha kutarisa kufambira mberi kwebromophenol blue dye pamberi, iyo inoratidza kupera kwe electrophoresis. Kune majiroji ane huwandu hwe10-12%, 80-90 maminetsi anowanzo kukwana; zvisinei, nokuda kwe15% gels, ungada kuwedzera nguva yekumhanya zvishoma.
Kuronga Nguva: Kuziva Nguva Yekumira
Nguva ndechimwe chinhu chakakosha mu gel electrophoresis. Kumhanyisa gel kwenguva yakarebesa kana kupfupika kwenguva kunogona kutungamirira kune suboptimal kupatsanurwa. Kutama kwebromophenol dhayi yebhuruu chiratidzo chinobatsira: kana ichisvika pasi pejeri, inowanzova nguva yekumisa kumhanya. Pamajelisi akajairwa, akadai se10-12%, nguva ye electrophoresis inosvika 80-90 maminetsi inowanzokwana. Kune yakakwira muzana gels, senge 15%, nguva yekumhanya inofanirwa kuwedzerwa kuti ive nechokwadi chekuparadzaniswa kwakakwana kwemapuroteni.
Buffer Management: Kushandisazve uye Kugadzirira Mabhafa
Electrophoresis buffer inogona kushandiswazve ka1-2, zvichienderana nemamiriro emurabhoritari yako. Nekudaro, kune yakanyanya mhedzisiro, zvinokurudzirwa kugadzirira nyowani 10x buffer uye kuidzikisa usati washandisa. Izvi zvinovimbisa kuti buffer inochengetedza kushanda kwayo, zvichiita kuti iwedzere kuvimbika electrophoresis mhinduro.
Beijing Liuyi Biotechnology gel electrophoresis zvigadzirwa
Nekunyatso kudzora sampuli vhoriyamu, voltage, uye electrophoresis nguva, unogona zvakanyanya kunatsiridza iko kurongeka uye kuberekazve kweiyo gel electrophoresis mhinduro. Kushandisa aya akanakisa maitiro mubasa rako rerabhoritari kuchakubatsira iwe kuwana akajeka uye akajeka mabhendi, zvichitungamira kune data riri nani rekuongorora kuzasi.
Kana iwe uine dzimwe nzira dzakanaka dzekugadzirisa gel electrophoresis kuyedza, unogamuchirwa kukurukura nesu!
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ine hunyanzvi mukugadzira zviridzwa zve electrophoresis kweanopfuura makore makumi mashanu nechikwata chedu chehunyanzvi chehunyanzvi uye R&D centre. Isu tine yakavimbika uye yakakwana yekugadzira mutsara kubva padhizaini kusvika pakuongorora, uye imba yekuchengetera, pamwe nerutsigiro rwekushambadzira. Zvigadzirwa zvedu zvikuru ndeElectrophoresis Cell (tangi/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Isu tinopawo zvishandiso zvelab sePCR instrument, vortex mixer uye centrifuge ye laboratori.
Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu. Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.
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Nguva yekutumira: Aug-20-2024