Protein Blotting
Protein blotting, inonziwo western blotting, kuendesa mapuroteni kune yakasimba-chikamu membrane inotsigira, inzira ine simba uye yakakurumbira yekuona uye kuzivikanwa kwemapuroteni.
Kazhinji, mapuroteni ekudzima kufambiswa kwebasa anosanganisira kusarudzwa kweiyo nzira yakakodzera, zvishandiso, membrane, buffer, uye mamiriro ekutamisa. Kamwe mapuroteni akaiswa pa membrane, anowanikwa pakuona, kuona, uye kuongorora.
Liuyi Biotechnology inopa masaizi akasiyana eiyo vertical electrophoresis system iyo inogona kuzadzisa zvaunoda kuedza. Mamwe eLiuyi's vertical electrophoresis masero (matangi/makamuri) anoenderana neyedu electrode gungano rekutamisa mapuroteni molecule kubva gel kuenda membrane kumadokero blotting kuyedza.Thevertical electrophoresis systemmodelDYCZ-24DN, DYCZ-25DuyeDYCZ-25Einoenderana neiyo tank kutamisa system modhiDYCZ-40D, DYCZ-40GuyeDYCZ-40F. Misiyano mikuru pamusoro pematangi aya saizi yetangi yebhafa vhoriyamu, uye saizi yekukanda gel. Thevertical electrophoresis systemmodelDYCZ-25Dine dhizaini yekupisa yekupisa yekutonhodza zvirinani panguva yekumhanya gels.
Blotting Method
Kune nzira dzakasiyana-siyana dzekufambisa, asi nzira inonyanya kufarirwa ndeye electrophoretic transfer, nokuti inokurumidza uye inoshanda kupfuura dzimwe nzira. Pane nzira nhatu dzinogona kutamisa mapuroteni kubva kuSDS-PEJI kana gel kuenda ku membrane: kutamisa tangi, semi-yakaoma kutamisa uye kuendesa kwakaoma.Beijing liuyiBiotechnologyanezvigadzirwa zvekutamisa tank, semi-dry transfer, uye tizivise zvimwe zvakawanda nezvazvokutamisa tangiuyesemi-dry transfer.
Matangi ekutamisa masisitimu - gels uye membranes akanyudzwa mukati mekutamisa buffer mumatangi; masisitimu aya anobatsira kune akawanda enguva dzose basa reprotein, kune inoshanda uye yakawanda mapuroteni kutamisa, uye nekutamisa mapuroteni eese saizi. Matangi ekufambisa masisitimu anopa kuchinjika kwakanyanya mukusarudza magadzirirwo emagetsi, nguva dzekudzima, uye sarudzo dzekutonhora.
Semi-dry systems - gels uye membranes zvakaputirwa pakati pe-buffer-saturated filter papers iyo inosangana zvakananga neplate electrodes; masisitimu aya anowanzo kuve nyore kumisikidza pane matangi masisitimu uye anobatsira kana yakakwirira-kuburikidza ichidikanwa uye yakawedzerwa nguva yekufambisa isingadikanwi, kana kana discontinuous buffer masisitimu achishandiswa. Active kutonhora sarudzo dzinogumira ne semi-dry blotting.
DYCP-40C
BeijingLiuyi Biotechnology inogona kupa matangi ekufambisa masisitimu uye semi-yakaoma masisitimu iwe yaunosarudza. MuenzanisoDYCZ-40D, DYCZ-40F,uyeDYCZ-40Gndiwo masisitimu edu ekufambisa matangi; muenzanisoDYCP-40CuyeDYCP-40Eari semi-dry systems.
Liuyi brand ine makore anopfuura makumi mashanu nhoroondo muChina uye kambani inogona kupa yakagadzikana uye yemhando yepamusoro zvigadzirwa pasi rese.
Kuti uwane rumwe ruzivo nezvedu, ndapota taura nesu neemail[email yakachengetedzwa], kutengesa01@ly.com.cn
Nguva yekutumira: Kubvumbi-08-2022