Protein electrophoresis inzira yemurabhoritari inoshandiswa kupatsanura uye kuongorora mapuroteni zvichienderana nekukura kwawo uye kubhadharisa.,kupa hwindo mukati mekuoma kweprotein musanganiswa. Iyi nzira inotora mukana wekuti mapuroteni ane machaji akasiyana nekuda kwekuumbwa kwawo kweamino acid. Kana munda wemagetsi ukaiswa kune sampuli yeprotein, mapuroteni anofamba kuburikidza nejeri matrix kana mhinduro pamitengo yakasiyana zvichienderana nechaji-kusvika-huwandu hwehuwandu.
Mhando mbiri dzakajairika dzeprotein electrophoresis ndeye polyacrylamide gel electrophoresis (PEJI) uye agarose gel electrophoresis. MuPEJI, polyacrylamide gel inoshanda sematrix iyo mapuroteni anofamba. Iyo gel's porous nature inoderedza mapuroteni makuru kupfuura maduku, zvichiita kuti kuparadzaniswa kunobva pakukura. Sodium dodecyl sulfate (SDS) inowanzoshandiswa pamwe chete nePEJI kuti denature mapuroteni uye neutralize mhosva dzavo, kuitira kuti kuparadzana kunova kunyanya kutsamira saizi. Musiyano uyu unozivikanwa seSDS-PAGE.
Protein electrophoresis ine maapplication mundima dzakasiyana, senge molecular biology, biochemistry, proteomics, uye kiriniki diagnostics. Inowanzo shandiswa kuongorora kuumbwa kweprotein, kuyera mapuroteni ekutaura mazinga, kuona post-shanduro shanduko, uye kuenzanisa mapuroteni maprofayili pakati pemasampuli akasiyana..
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Nguva yekutumira: Aug-15-2023