Gel haina kugadzika zvakanaka
Nyaya: Iyo gel ine maitiro kana kuti haina kuenzana, kunyanya mu-high-concentration gels panguva yechando inotonhora yechando, apo pasi pejeri rinoparadzanisa rinoratidzika wavy.
Solution: Wedzera huwandu hwema polymerizing agents (TEMED uye ammonium persulfate) kukurumidza kugadzirisa maitiro. Ita shuwa kuti magirazi egirazi akachena kudzivirira zvisaririra kubva kune apfuura gels.
Gel is Fragile
Nyaya: Low-concentration gels (inoshandiswa kuhombe molecular weight protein) inoputsika panguva yekubata.
Mhinduro: Bata gel zvinyoro-nyoro mukati mekuita. Mumamiriro ekutonhora, wedzera zvishoma huwandu hwema polymerizing agents.
Gel Haina Kugadzika
Solution: Mukupisa kwakadzika, wedzera huwandu hwe polymerizing agents. Ita shuwa kuti ammonium sulfate yakagadziridzwa nyowani. Kana dambudziko rikaramba riripo, gadzirirazve kugadzirisa kwebhafa.
Mazhinji Streaks uye Smears paGel Mushure meElectrophoresis
Mhinduro: Iva nechokwadi chekuti gel mhinduro yakachena uye yakanyatsosanganiswa. Dzora kushandiswazve kwe electrophoresis buffer kana kudzivirira kushandisa zvakare zvachose. Kana uchichengetedza pabuffer, shandisa buffer nyowani mukamuri yemukati uye shandisazve buffer mukamuri yekunze.
Nguva Yakashata Yekugadzika Gel
Nyaya: Gels dzakagadzirwa kumba dzinowanzoiswa mukati me30 maminetsi kusvika paawa, nepo makiti ekutengesa anoiswa mukati me10-20 maminetsi. Kana kuseta kuchinonoka, iyo polymerizing agent dosage inogona kunge isina kukwana. Kana kuisa kwakanyanyisa, kuyerwa kunogona kunge kwakanyanyisa, zvichiita kuti gel rive rakaoma uye rinowanzoputika, uye kuwedzera njodzi yekupisa panguva ye electrophoresis.
Solution: Gadzirisa huwandu hwema polymerizing agents zvichienderana nemamiriro chaiwo. Nguva yekuisa gel inonyanya kukonzerwa nekushisa.
Beijing Liuyi Biotechnology Co., Ltd ibhizinesi rehurumende retekinoroji rinonyanya kugadzira biochemistry uye molecular biology laboratory analytical zviridzwa. Liuyi ane hunyanzvi mukugadzira zviridzwa zve electrophoresis kweanopfuura makore makumi mashanu neayo processional tekinoroji timu uye R&D centre. Iyo ine yakavimbika uye yakakwana yekugadzira mutsara kubva pakugadzira kusvika pakuongorora, uye imba yekuchengetera, pamwe nerutsigiro rwekushambadzira. Zvigadzirwa zvikuru ndeElectrophoresis Cell (tangi / chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc .. Maererano nezvinodiwa nevatengi, Inogona kupa basa rakagadzirirwa.
Kune marudzi akasiyana-siyana emagetsi e electrophoresis matangi eprotein electrophoresis yekuongorora uye kuzivikanwa kweprotein samples ne polyacrylamide gel electrophoresis, uye zvakare yekuyera sampuli 'mamorekuru huremu, kuchenesa masampuli uye kugadzirira masampuli. Zvigadzirwa izvi zvese zvinogamuchirwa mumusika wepamba uye mhiri kwemakungwa.
Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu. Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.
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Nguva yekutumira: Jul-29-2024