Pano tichatsanangura maitiro ekuita agarose gel electrophoresis nemuongorori wedu muLiuyi Biotech's lab.
Tisati taedza, tinofanirwa kutarisa zvishandiso, mareagents, uye zvimwe zvinhu zvekuedza uye zvishandiso zvatinoda.
Kugadzirira kwemidziyo yekuedza uye zvinhu
Zvishandiso zve agarose gel electrophoresis
Horizontal electrophoresis cell (tangi / kamuri), yepakati uye yakaderera voltage electrophoresis magetsi, gel mufananidzo & yekuongorora system.
Beijing liuyi Biotechnology Co., Ltd (Liuyi Biotech) inopa mhando dzakasiyana dzeyakachinjika electrophoresis sero (tangi/chamber) uye magetsi pamwe neiyo gel zvinyorwa system. Iyo modhi yeDYCP-31 yakatevedzana zvigadzirwa ndeye electrophoresis, uye DYY akatevedzana zvigadzirwa ndiyo electrophoresis magetsi emagetsi. Iyo WD-9413 yakatevedzana zvigadzirwa ndiyo gel zvinyorwa zvinyorwa. Kubva padiki gel saizi 60 × 60mm kusvika gel hombe 250 × 250mm, tinogona kuzadzisa zvaunoda zvakasiyana zvesaizi yegel. MuenzanisoDYCP-32Cinogona kuita kuti gel isvike saizi 250 × 250mm. TheDYY-6Cndiyo magetsi edu. Inotsigira kubuda kwe400V, 400mA, 240W, chinova chigadzirwa chedu chinoshandiswa nevatengi vedu. WD-9413BGel Image & Analysis System inoshandiswa kuongorora nekutsvaga gel, mafirimu, uye mablots mushure mekuedza kwe electrophoresis. Icho chinhu chakakosha chine ultraviolet mwenje sosi yekuona uye kutora mafoto gels akasvibiswa nedhayi dzefluorescent senge ethidium bromide, uye ine chena mwenje sosi yekuona uye kutora mafoto gels akasvibiswa nedhayi senge coomassie brilliant blue.
Iyo reagents yeagarose gel electrophoresis
1.The medium: agarose gel
2.The buffer: TAE (Tris-acetate, EDTA, glacial acetic acid) uye TBE (Tris-borate, EDTA, boric acid).
3.Mushonga wekutakura: 6 × DNA Loading Buffer (Special for DNA sample: EDAT, glycerine, Xylene cyanol uye bromophenol blue)
The Dye
Iyo fluorescent dhayi seEB, Gelred, goldview, GenGreen, GenView, SYBRGreen
TheConsumables
Sterilization pipette tips (10μL), matipi epipette (200μL), matipi epipette (1000μL), 200μL\500μL\1.5ml centrifuge chubhu.
DNA Marker
Yakagadzirirwa maererano nehuremu hwema molecular kuti iongororwe.
Iyo Gel inomhanya uye tarisa
Kutanga, tinoda kugadzirira gel agarose. Kuwanda kweagarose mujeri kuchaenderana nehukuru hwezvimedu zveDNA zvichapatsanurwa, nemajeri mazhinji ari pakati pe0.5% -2%. Kutora yeduDYCP-31DNsemuenzaniso, Unogona kukanda diki gel, yakafara gel, refu gel uye square gel zvinoenderana nezvinodiwa zvekuyedza. Sarudza tireyi yegel yaunoda, woisa muzinga, wodira gel rinopisa mumudziyo wekukanda gel.
Zvadaro, mushure mekunge gel rasimba, bvisa muzinga zvakanaka uye zvinyoro, uye woisa tireyi yegel mubhafa tangi. Dururira mushonga webuffer mubhafa tangi uye ita kuti gel rese rinyudzwe mubuffer. Takura masampuli mumatsime ane pipette yakajairika. Batanidza iyoDYCP-31DNne electrophoresis simba rekupa nenzira kwayo, uye isa zvigadziro zvekushandisa gel.
Fungidzira yako DNA zvimedu mune UV transilluminator.
Amushure mekumhanyisa gel, unogona kushandisa yedu gel mufananidzo & yekuongorora system modhiWD-9413Bkutarisa, kuongorora uye kutora mapikicha ejeri. Liuyi Biotech inopawo UV transilluminator (UV Analyzer) kuona iyo gel. Tine dema-bhokisi mhando UV transilluminator (UV Analyzer) modhiWD-9403A, 9403C, WD-9403F, inotakurika UV transilluminator (UV Analyzer) modhiWD-9403Buye kubata UV transilluminator (UV Analyzer)WD-9403Enokuti unosarudza.
Mufananidzo we gel post electrophoresis
Liuyi brand ine makore anopfuura makumi mashanu enhoroondo muChina uye kambani inogona kupa yakagadzikana uye yemhando yepamusoro zvigadzirwa pasi rese. Kupfuura makore ebudiriro, yakakodzera sarudzo yako!
Kuti uwane rumwe ruzivo nezvedu, ndapota taura nesu neemail[email yakachengetedzwa], [email yakachengetedzwa].
Nguva yekutumira: Kubvumbi-29-2022