Electrophoresis Kugadzirira uye Sample Application paAcetate Cellulose Membrane

Preprocessing Acetate Cellulose Membrane

Kucheka Membrane:

Cheka iyo acetate cellulose membrane kuita saizi chaiyo zvichienderana nehuwandu hweakapatsanurwa samples, kazhinji 2.5cmx11cm kana 7.8cmx15cm.

Kumaka Sample Application Line:

• Parutivi rusina kupenya rwe membrane, zvinyoro nyora mutsara wekushandisa sampuli nepenzura.
• Nzvimbo yemutsara wekushandisa inogona kusarudzwa 2-3cm kubva kune imwe mugumo we membrane kana dzimwe nguva pedyo nechepakati.
• Nzvimbo yemutsara wekushandisa inowanzogadziriswa zvichienderana nekutanga electrophoresis miedzo.

Kunyura muElectrode Buffer Solution:

• Mundiro isina kudzika kana tsika ndiro, dururira mu electrode buffer solution.
• Nyatsoyangarara membrane pamusoro pechigadziriso che electrode buffer, uchiona kuti rutivi rusina kupenya rwakatarisa pasi.
• Sezvo pasi pe membrane inotora mhinduro ye electrode buffer, inonyura zvishoma nezvishoma kusvika yazara pasi.

Kubvisa uye Kubvisa Yakawandisa Liquid:

• Mushure mekunge membrane yapinda mumhinduro ye electrode buffer, shandisa blunt forceps kuti unyatsoibvisa.
• Sandwich iyo membrane pakati pematanho maviri epepa resefa kuti itore yakawandisa electrode buffer mhinduro, kudzivirira kuoma kwakanyanya.
• Kana nzvimbo chena yakavharika ichionekwa pa membrane, zvichiratidza kuoma zvakanyanya, nyudza zvakare membrane mu electrode buffer solution uye tora kusvika padanho rakakodzera nesefa bepa.

Sample Application process

Kusarudza uye Kushanda paSample Application Line:

Isa sampuli pamwe chete nemutsara wekushandisa mutsara padivi risiri-kupenya re membrane, kazhinji mune mutsara pateni pane yekunongedza-yakafanana application.

Qualitative Analysis Sample Application:

• Shandisa capillary chubhu (ine dhayamita ye0.5 ~ 1.0mm) kana molds yemhando yekuongorora sampuli yekushandisa.
• Panguva yekuongorora kwemhando, nyudza sampuli uye "kuidhinda" pamwe chete nemutsara wekushandisa mutsara.

Quantitative Analysis Sample Application:

• Shandisa sirinji ine microliter yehuwandu hwekuongorora sampuli yekushandisa.
• Paunenge uchishandisa capillary chubhu kana microliter sirinji, zvinyatso fambisa kumusoro uye pasi kana kuruboshwe uye kurudyi uchitevedza sampuli yekushandisa mutsara kusvikira yafanotemerwa vhoriyamu yesampuli yaiswa.

Kudzora Sample Line Dimensions:

• Mushure mekushandisa sampuli yega yega pa membrane, kureba kwemutsara mutsara kazhinji 1.5cm, nehupamhi husingadariki 4mm.
• Nhambwe iri pakati pemitsara yemuenzaniso uye pakati pemutsara wemuenzaniso uye mupendero wakareba we membrane inowanzoita 3 ~ 5mm.

Kugadzirisa Sample Volume:

Huwandu kana vhoriyamu yemuenzaniso yakashandiswa inosiyana zvakanyanya zvichienderana nezvinhu zvakaita seyekumisikidza sampuli, kusvibisa, uye nzira dzekuongorora.

Sample Volume panguva Quantitative Analysis:

Pakuongorora kwehuwandu, iyo yakashandiswa sampuli vhoriyamu pane imwe neimwe sendimita yemuenzaniso wekushandisa mutsara kazhinji 0.1-0.5μl, yakaenzana nehuwandu hwe5-1000μg protein sample.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ine hunyanzvi mukugadzira zviridzwa zve electrophoresis kweanopfuura makore makumi mashanu nechikwata chedu chehunyanzvi chehunyanzvi uye R&D centre. Isu tine yakavimbika uye yakakwana yekugadzira mutsara kubva padhizaini kusvika pakuongorora, uye imba yekuchengetera, pamwe nerutsigiro rwekushambadzira. Zvigadzirwa zvedu zvikuru ndeElectrophoresis Cell (tangi/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc.

Liuyi Bilotechnology inogadzira imwe modhi ye electrophoresis tank inodaidzwa kuti cellulose acetate membrane elctrophoresis tank DYCP-38C inogona kuchinjirwa kuongororwa kwemhando dzakasiyana dzehemoglobin, kusanganisira idzo dzine chekuita nechirwere chesickle cell (SCD). Iyo itsika uye yehupfumi nzira ye hemoglobin electrophoresis.

Kana iwe uchifarira nzira iyi yakapusa uye yehupfumi ye electrophoresis, ndapota shanyira chinyorwa chinotevera kune maprotocol uye nhungamiro yehemoglobin electrophoresis inopihwa nevayedza vedu.

Kuedza kupatsanura serum protein neCellulose Acetate Membrane Electrophoresis

Iyo Beijing Liuyi biotechnology yanga yakatarisana nekugadzira zvigadzirwa zve electrophoresis kweanopfuura makore makumi mashanu nhoroondo muChina uye kambani inogona kupa yakagadzikana uye yemhando yepamusoro zvigadzirwa pasi rese. Kupfuura makore ebudiriro, yakakodzera sarudzo yako!

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