Comet Assay (Single Cell Gel Electrophoresis, SCGE) inyanzvi uye inokurumidza nzira inonyanya kushandiswa kuona kukuvara kweDNA uye kugadzirisa mumaseru ega. Zita rokuti "Comet Assay" rinobva kune chimiro che comet-like chimiro chinowanikwa mumigumisiro: nucleus yesero inoumba "musoro," apo zvidimbu zveDNA zvakakanganiswa zvinotama, zvichiita "muswe" wakafanana nekometi.
Principle
Nheyo yeComet Assay yakavakirwa pakufamba kwezvimedu zveDNA mumunda wemagetsi. DNA isina kujeka inoramba iri mukati memukati mesero, nepo DNA yakakuvadzwa kana kupatsanurwa ichienda kune anode, ichigadzira "muswe" wecomet. Kureba uye kusimba kwemuswe zvakananga kuenzana nehupamhi hwekukuvadzwa kweDNA.
Maitiro
- Kugadzirira Sero: Masero anofanirwa kuongororwa anosanganiswa neagarose yakaderera-kunyungudika uye anopararira pamasiraidhi emaikorosikopu kuti aite jira rakafanana.
- Sero Lysis: Masiraidhi anonyudzwa mune lysis mhinduro yekubvisa sero membrane uye nukireya membrane, ichiburitsa DNA.
- Electrophoresis: Masiraidhi anoiswa mukamuri ye electrophoresis pasi pe alkaline kana mamiriro asina kwaakarerekera. Zvimedu zveDNA zvakakuvadzwa zvinotamira kune yakanaka electrode pasi pesimba remagetsi.
- Staining: Mushure me electrophoresis, masiraidhi akasvibiswa nedhayi refluorescent (semuenzaniso, ethidium bromide) kuti uone DNA.
- Microscopic Analysis: Uchishandisa fluorescence maikorosikopu kana nyanzvi software, maumbirwo ecomet anoongororwa, uye zvimiro zvakaita sehurefu hwemuswe uye kusimba zvinoyerwa.
Mufananidzo kubva kubiorender
Data Analysis
Mhedzisiro kubva kuComet Assay inoongororwa zvichibva pane akati wandei akakosha paramita:
- Muswe Kureba: Inomiririra kureba kwainotama DNA, zvichiratidza kukura kwekukuvadzwa kweDNA.
- Muswe DNA Content: Chikamu cheDNA chinopinda mumuswe, chinowanzo shandiswa seyero yehuwandu hwekukuvara kweDNA.
- Olive Muswe Nguva (OTM): Inosanganisa kureba kwemuswe uye muswe DNA zvirimo kuti ipe chiyero chakakwana chekukuvadza kweDNA.
Applications
- Genotoxicity Zvidzidzo: Iyo Comet Assay inoshandiswa zvakanyanya kuongorora mhedzisiro yemakemikari, zvinodhaka, uye nemwaranzi pasero DNA, ichiita chishandiso chakakosha chekuongorora genotoxicity.
- Environmental Toxicology: Inobatsira mukuongorora kukanganisa kwezvinosvibisa kwezvakatipoteredza paDNA yezvipenyu, ichipa ruzivo rwekuchengetedza ecosystem.
- Ongororo yezvekurapa uye yekiriniki: Iyo Comet Assay inoshandiswa mukudzidza DNA kugadzirisa masisitimu, cancer, uye zvimwe zvirwere zvine chekuita neDNA. Inoongororawo maitiro ekurapa cancer senge radiotherapy uye chemotherapy paDNA.
- Food and Agriculture Sciences: Inoshandiswa kuongorora kuchengetedzeka kwemishonga yezvipembenene, zvinowedzera chikafu, uye zvimwe zvinhu, uye kudzidza mhedzisiro yazvo muchetura mumhando dzemhuka.
Zvakanakira
- High Sensitivity: Inokwanisa kuona yakaderera mazinga eDNA kukuvara.
- Nyore Kushanda: Nzira yacho yakatwasuka, ichiita kuti ive yakakodzera kutariswa kwepamusoro-soro.
- Wide Application: Inogona kuiswa kune akasiyana siyana masero, kusanganisira mhuka uye chirimwa maseru.
- Quantification Matambudziko: Ndichiri kupa dhata remhando yekukuvara kweDNA, kuongororwa kwehuwandu kunovimba zvakanyanya nesoftware uye maitiro ekuongorora mifananidzo.
- Experimental Conditions: Mhedzisiro inogona kupesvedzerwa nezvinhu zvakaita se electrophoresis nguva uye pH, zvinoda kunyatso kudzora mamiriro ekuyedza.
Kuganhurirwa
Iyo Comet Assay chishandiso chakakosha mukutsvagisa biomedical, sainzi yezvakatipoteredza, uye kuvandudzwa kwezvinodhaka nekuda kwekushanduka kwayo uye kunzwa kwakanyanya mukuona kukuvadzwa nekugadzirisa kweDNA. Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology)inopa horizontal electrophoresis chamber yecomet assay. Kugamuchirwa kuti tibatane nesu kuti tikurukure nezveComet Assayprotocol.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ine hunyanzvi mukugadzira zviridzwa zve electrophoresis kweanopfuura makore makumi mashanu nechikwata chedu chehunyanzvi chehunyanzvi uye R&D centre. Isu tine yakavimbika uye yakakwana yekugadzira mutsara kubva padhizaini kusvika pakuongorora, uye imba yekuchengetera, pamwe nerutsigiro rwekushambadzira. Zvigadzirwa zvedu zvikuru ndeElectrophoresis Cell (tangi/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Isu tinopawo zvishandiso zvelab sePCR instrument, vortex mixer uye centrifuge ye laboratori.
Kana iwe uine chero chirongwa chekutenga chezvigadzirwa zvedu, ndapota usazeza kutibata nesu. Unogona kutitumira meseji paemail[email yakachengetedzwa]kana[email yakachengetedzwa], kana kuti tapota kutifonera panhamba dzinoti +86 15810650221 kana kuwedzera Whatsapp +86 15810650221, kana Wechat: 15810650221.
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Nguva yekutumira: Sep-20-2024