Cellulose Acetate Membrane Electrophoresis

Chii chinonzi Cellulose Acetate Membrane Electrophoresis?

Cellulose Acetate Membrane Electrophoresis imwe mhando yemaitiro e electrophoresis anoshandisa cellulose acetate membrane sechinhu chinotsigira midhiya yekuyedza.

Cellulose Acetate imhando yeacetate ye cellulose iyo acetylated kubva ku cellulose's hydroxyl. Kana ichinyungudika mune organic solution senge acetone, inogona kuvezwa kune homogeneous uye yakanaka firimu ine micro-pores. Ukobvu hwefirimu hwakatenderedza 0.1-0.15mm iyo yakakodzera kuyedza.

Zvakanakira zve cellulose acetate membrane

Kuenzanisa nefliter bepa, ine zvinotevera zvakanakira:

1.Good effect yekuparadzana. Kune mapuroteni samples, cellulose acetate membrane inotora mashoma ayo, mushure medhayi, inogona kudzima zvachose kubva kumashure kwemavara. Mabhandi edhayi akajeka kwazvo, saka anosimudzira kurongeka kwehuwandu hwekutsunga.
2.Fast uye kuchengetedza nguva. Iyo cellulose acetate membrane haina hydyophilic pane fliter paper, saka electro-osmosis kushanda ishoma pane fliter bepa, uye magetsi emagetsi anonyanya kuitiswa nemasamples anogona kupfupisa nguva yekuparadzanisa uye kushomeka nguva ye electrophoresis. Kazhinji, iyo cellulose acetate membrane electrophoresis nguva inenge 45-60minutes. Iyo yese maitiro e electrophoresis kusanganisira kudhaya uye decoloration inongoda maminitsi makumi mapfumbamwe.
3.Kunzwa kwakanyanya uye mashoma masampuli anoshandiswa. Inongoda 2 μL serum mu serum protein electrophoresis.
4.Wide application. Mamwe mapuroteni haasi nyore kuparadzanisa pamapepa electrophoresis, saAlpha-fetoprotein, bacteriolytic enzyme, insulini, uye histone etc. Asi zviri nani kushandisa cellulose acetate membrane electrophoresis kuti iparadzanise.
5.Easy kuchengeta uye quantification. Mushure mekudhaya cellulose acetate membrane electrophoresis samples, nyudza masampuli mumusanganiswa weglacial acetic acid uye ethanol kuti ugadzire ndiro inoonekera yakaoma inochengetwa kwenguva yakareba.

Nekuti iyo cellulose acetate membrane electrophoresis iri nyore kushanda, ikozvino inoshandiswa zvakanyanya mukuongorora uye kuyedza plasma protein, lipoprotein, glycoprotein, Alpha-fetoprotein, enzyme, polypeptide, nucleic acid uye mamwe biomacromolecule.

Kugadzirira kweCellulose Acetate Membrane Electrophoresis

Midziyo inoshandiswa inosanganisira yakaderera magetsi magetsi, horizontal electrophoresis tank. Iyo inotsigira midhiya ndeye cellulose acetate membrane.

The reagents:

1.pH8.6 barbitol buffer mhinduro: Diethyl barbituric acid, Diethyl sodium pentobarbital;
2.Stain:Ponceau S, Trichloroacetic;
3.TBS / T kana PBS / T: 95% ethanol, glacial acetic acid;
4.Kuchenesa Kugadzirisa: anhydrous ethanol, glacial acetic acid.

Beijing Liuyi Biotechnology Co., Ltd ine yakakura system ye cellulose acetate membrane electrophoresis. Iyo modhi DYCP-38C ikamuri yakatetepa yebepa uye inotsvedza electrophoresis, uye cellulose acetate membrane electrophoresis. Muviri mukuru wakaumbwa, hapana kuvuza chiitiko. Iyo magetsi emhando yeDYY-2C, DYY-6C, DYY-6D, DYY-8C , DYY-10C uye DYY-12 inogona kupa simba reDYCP-38C. Kazhinji, mutengi anofarira kusarudza modhi DYY-6C semagetsi ekupa DYCP-38C.

Sechigadzirwa chinodiwa cheDYCP-38C electrophoresis tank, Liuyi anopawo cellulose acetate membrane. Kune matatu akajairwa saizi ye membrane, 7 * 9cm, 2 * 8cm uye 12 * 8cm, uye isu tinogona kupa yakasarudzika saizi ye cellulose acetate membrane sezvinodiwa zvako zvakare.

Zvinyorwa pamusoro pekuedza:

1.Kuisa sampuli padivi rakashata: 1-2UL yakakodzera
2.Constant current electrophoresis: simba razvino 0.4 ~0.6m A/cm
3.pH8.6 barbitol buffer mhinduro:Ionic simba 0.06
4.Dyeing nguva: 5 maminitsi zvakakwana
5.Kuchengetedza: isa mepu yakaoma elecrtophoresis mukuchenesa mhinduro ye 10-15 maminitsi, uye woitora kunze uye kuinamatira pagirazi rakachena, mushure mokunge yaoma, ichava mepu yefirimu yakajeka.

Liuyi brand ine makore anopfuura makumi mashanu nhoroondo muChina uye kambani inogona kupa yakagadzikana uye yemhando yepamusoro zvigadzirwa pasi rese.

Kuti uwane rumwe ruzivo nezvedu, ndapota taura nesu neemail[email yakachengetedzwa], [email yakachengetedzwa].