1. Kuronga
Gel electrophoresis rakakamurwa vertical mhando (kusanganisira column gels uye slab gels) uye chinjikira mhando (kunyanya slab gels) (Figure 6-18). Kazhinji, kupatsanurwa kwakatwasuka kwakakwirira zvishoma kune yakatwasuka, asi yakatwasuka gel kugadzirira ane zvinenge zvakanakira mana: pane kutsigirwa pasi rose gel, achibvumira kushandiswa low-concentration agarose; zvinokwanisika kugadzirira agarose gel mahwendefa ezvinyorwa zvakasiyana; kugadzirira gel uye sampuli kurodha zviri nyore; iyo electrophoresis chamber iri nyore kugadzira uye inodhura-inoshanda. Sezvo horizontal electrophoresis inoitwa neagarose gel plate yakanyura zvakazara pamusoro pe1mm pazasi pepamusoro peiyo electrophoresis buffer, inonziwo undermerged electrophoresis.
Electrophoresis Tangi DYCP-31DN yeagarose gel electrophoresis
2.Buffer System
Mukuparadzanisa nucleic acid, masisitimu mazhinji anotora anoenderera masisitimu. Anowanzo shandiswa electrophoresis buffers anosanganisira TBE (0.08mol/L Tris·HCl, pH 8.5, 0.08mol/L boric acid, 0.0024mol/L EDTA) buffer uye THE (0.04mol/L Tris·HCl, pH 7.8, 0.02mol/L) sodium acetate, 0.0018mol/L EDTA) buffer. Aya mabuffers anowanzo gadzirirwa se10x stock solution uye anonatsirwa kune inodiwa yekumisikidza kana ichishandiswa. Kutama kwemazinga emutsetse uye denderedzwa DNA muagarose gel inosiyana nebuffer inoshandiswa. MuTHE buffer, mwero wekufamba kwemutsetse weDNA wakakura kupfuura weDNA yedenderedzwa, ukuwo muTBE buffer, zvakapesana ichokwadi.
3.Kugadzirira kweAgarose Gel
(1) Kugadzirira kweHorizontal Agarose Gel
(a) Gadzirira kusungirirwa kunodiwa kweagarose gel uchishandisa 1x electrophoresis buffer.
(b) Pisa agarose kuti ipedze kunyungudika mubhavhu remvura iri kufashaira, pamagnetic stirrer, kana mumicrowave. Tonhodza mhinduro yeagarose kusvika 55 ° C uye wobva wawedzera ethidium bromide (EB) dhayi kune yekupedzisira kusanganiswa kwe 0.5 μg/ml.
(c) Nama micheto yegirazi kana acrylic ndiro nekadiki kejeri yeagarose, wedzera muzinga, woisa mazino emuzinga anenge 0.5 ~ 1.0 mm pamusoro pendiro.
(d) Dururirai yakanyungudutswa agarose gel solution nguva dzose mugirazi kana acrylic plate mold (ukobvu hunoenderana nehuwandu hwemuenzaniso weDNA), kudzivisa kuiswa kwemhepo inoputika. Rega zviome zvakasununguka pakupisa kwekamuri.
(e) Nyatsobvisa muzinga mushure mekunyatsosimba. Wedzera huwandu hwakakodzera hwe electrophoresis buffer kune gel tank, kuve nechokwadi chekuti gel plate yakanyura inenge 1 mm pasi pepamusoro pe electrophoresis buffer.
(2) Kugadzirira kweVertical Agarose Gel
(a) Bvisa girisi kana zvakasara kubva mumagirazi egirazi nekugeza ne ethanol.
(b) Isai maplate emuchadenga pakati pemadhamu emberi neshure, gadzirisai mipendero yemaplate emuchadenga nemadhamu ekumberi nekumashure, woasimbisa nemagetsi.
(c) Wedzera 2% agarose mu 1x buffer pakati pemicheto yespacer plates kuti uite 1 cm yakakwirira agarose plug pazasi pekamuri yekukanda gel.
(d) Dururira gel yakanyungudutswa agarose pane yaunoda, yakagadzirirwa mu 1x buffer, mukamuri yegel kusvika 1 cm pasi pepamusoro.
(e) Pinza muzinga, uchinzvenga kuteya mabhuru emhepo pasi pemazino emuzinga. Dzimwe nguva, wrinkles inogona kuoneka pamazino emuzinga panguva yekutonhodza kweagarose gel; mumamiriro ezvinhu akadaro, ingowedzera imwe agarose yakanyunguduka kumusoro kuti isimbise.
(f) Bvisa muzinga. Kuti udzivise kudonha kwebuffer munzvimbo yekurodha, isa chisimbiso pakati peagarose gel plate uye electrophoresis chamber ne2% agarose uye wedzera huwandu hunodiwa hwebuffer.
(g) Wedzera 1x electrophoresis buffer kukamuri yegel.
(h) Nyatsoisa DNA samples pane agarose gel pasi pe buffer.
Mamwe ruzivo nezve ruzivo rwekutanga nezve agarose gel electrophoresis, isu tichagovana svondo rinouya. Ndinoshuva kuti ruzivo urwu rubatsire pakuedza kwako.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ine hunyanzvi mukugadzira zviridzwa zve electrophoresis kweanopfuura makore makumi mashanu nechikwata chedu chehunyanzvi chehunyanzvi uye R&D centre. Isu tine yakavimbika uye yakakwana yekugadzira mutsara kubva padhizaini kusvika pakuongorora, uye imba yekuchengetera, pamwe nerutsigiro rwekushambadzira. Zvigadzirwa zvedu zvikuru ndeElectrophoresis Cell (tangi/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc.
Isu tave kutsvaga vanobatika, vese OEM electrophoresis tank uye vanogovera vanogamuchirwa.
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Nguva yekutumira: Zvita-07-2023